Literature DB >> 25609730

Discordance between Xpert MTB/RIF assay and Bactec MGIT 960 Culture System for detection of rifampin-resistant Mycobacterium tuberculosis isolates in a country with a low tuberculosis (TB) incidence.

Eiman Mokaddas1, Suhail Ahmad2, Hanaa S Eldeen3, Noura Al-Mutairi4.   

Abstract

Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10 Mycobacterium tuberculosis samples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpert assay. rpoB sequencing identified a silent (CTG521TTG) mutation in one isolate and a missense (GAC516TAC) mutation in another. The detection of rifampin resistance is imperfect with both the Xpert assay and MGIT system. Any discordant rifampin resistance results should be confirmed by sequencing of the rpoB gene.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 25609730      PMCID： PMC4365205          DOI： 10.1128/JCM.03412-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

1.  Performance comparison of four methods for detecting multidrug-resistant Mycobacterium tuberculosis strains.

Authors:  N M Al-Mutairi; S Ahmad; E Mokaddas
Journal:  Int J Tuberc Lung Dis       Date:  2011-01       Impact factor: 2.373

2.  Rifampin drug resistance tests for tuberculosis: challenging the gold standard.

Authors:  Armand Van Deun; Kya J M Aung; Valentin Bola; Rossin Lebeke; Mohamed Anwar Hossain; Willem Bram de Rijk; Leen Rigouts; Aysel Gumusboga; Gabriela Torrea; Bouke C de Jong
Journal:  J Clin Microbiol       Date:  2013-06-12       Impact factor: 5.948

3.  Isolation of Mycobacterium tuberculosis strains with a silent mutation in rpoB leading to potential misassignment of resistance category.

Authors:  María Alonso; Juan José Palacios; Marta Herranz; Ana Penedo; Angela Menéndez; Emilio Bouza; Darío García de Viedma
Journal:  J Clin Microbiol       Date:  2011-05-11       Impact factor: 5.948

4.  An evaluation of the Xpert MTB/RIF assay and detection of false-positive rifampicin resistance in Mycobacterium tuberculosis.

Authors:  Deborah A Williamson; Indira Basu; James Bower; Joshua T Freeman; Gillian Henderson; Sally A Roberts
Journal:  Diagn Microbiol Infect Dis       Date:  2012-07-20       Impact factor: 2.803

5.  Low-level rifampicin-resistant Mycobacterium tuberculosis strains raise a new therapeutic challenge.

Authors:  J van Ingen; R Aarnoutse; G de Vries; M J Boeree; D van Soolingen
Journal:  Int J Tuberc Lung Dis       Date:  2011-07       Impact factor: 2.373

6.  Diagnosis of tuberculosis and drug resistance: what can new tools bring us?

Authors:  F Drobniewski; V Nikolayevskyy; Y Balabanova; D Bang; D Papaventsis
Journal:  Int J Tuberc Lung Dis       Date:  2012-07       Impact factor: 2.373

7.  Molecular characterization of multidrug- and extensively drug-resistant Mycobacterium tuberculosis strains in Jiangxi, China.

Authors:  Xiaoliang Yuan; Tiantuo Zhang; Kazuyoshi Kawakami; Jiaxin Zhu; Hongtao Li; Jianping Lei; Shaohua Tu
Journal:  J Clin Microbiol       Date:  2012-05-02       Impact factor: 5.948

8.  Rapid molecular detection of tuberculosis and rifampin resistance.

Authors:  Catharina C Boehme; Pamela Nabeta; Doris Hillemann; Mark P Nicol; Shubhada Shenai; Fiorella Krapp; Jenny Allen; Rasim Tahirli; Robert Blakemore; Roxana Rustomjee; Ana Milovic; Martin Jones; Sean M O'Brien; David H Persing; Sabine Ruesch-Gerdes; Eduardo Gotuzzo; Camilla Rodrigues; David Alland; Mark D Perkins
Journal:  N Engl J Med       Date:  2010-09-01       Impact factor: 91.245

9.  Mycobacterium tuberculosis strains with highly discordant rifampin susceptibility test results.

Authors:  A Van Deun; L Barrera; I Bastian; L Fattorini; H Hoffmann; K M Kam; L Rigouts; S Rüsch-Gerdes; A Wright
Journal:  J Clin Microbiol       Date:  2009-09-16       Impact factor: 5.948

10.  Rifampin resistance missed in automated liquid culture system for Mycobacterium tuberculosis isolates with specific rpoB mutations.

Authors:  Leen Rigouts; Mourad Gumusboga; Willem Bram de Rijk; Elie Nduwamahoro; Cécile Uwizeye; Bouke de Jong; Armand Van Deun
Journal:  J Clin Microbiol       Date:  2013-06-12       Impact factor: 5.948
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  16 in total

1.  Reply to Murray et al., "Comparative Performance of BD MAX MDR-TB and Cepheid Xpert MTB/RIF Assays".

Authors:  Eiman M Mokaddas; Suhail Ahmad; Hanaa S Eldeen
Journal:  J Clin Microbiol       Date:  2019-08-26       Impact factor: 5.948

2.  Xpert MTB/RIF and Xpert MTB/RIF Ultra for pulmonary tuberculosis and rifampicin resistance in adults.

Authors:  David J Horne; Mikashmi Kohli; Jerry S Zifodya; Ian Schiller; Nandini Dendukuri; Deanna Tollefson; Samuel G Schumacher; Eleanor A Ochodo; Madhukar Pai; Karen R Steingart
Journal:  Cochrane Database Syst Rev       Date:  2019-06-07

3.  First insights into the phylogenetic diversity of Mycobacterium tuberculosis in Kuwait and evaluation of REBA MTB-MDR assay for rapid detection of MDR-TB.

Authors:  Noura M Al-Mutairi; Suhail Ahmad; Eiman Mokaddas; Sahal Al-Hajoj
Journal:  PLoS One       Date:  2022-10-20       Impact factor: 3.752

4.  Clinical implications of discrepant results between genotypic MTBDRplus and phenotypic Löwenstein-Jensen method for isoniazid or rifampicin drug susceptibility tests in tuberculosis patients.

Authors:  Ji Young Kang; Jung Hur; Shinyoung Kim; Sanghoon Jeon; Jaeha Lee; Youn Jeong Kim; Seok Chan Kim; Yeon Joon Park; Young Kyoon Kim; Hwa Sik Moon
Journal:  J Thorac Dis       Date:  2019-02       Impact factor: 2.895

5.  Performance Comparison of GeneXpert MTB/RIF and ProbeTec ET Tests for Rapid Molecular Diagnosis of Extrapulmonary Tuberculosis in a Low TB/MDR-TB Incidence Country.

Authors:  Eiman Mokaddas; Suhail Ahmad; Hanaa Eldeen
Journal:  Med Princ Pract       Date:  2021-02-16       Impact factor: 1.927

6.  Validation of the FluoroType MTBDR Assay for Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Complex Isolates.

Authors:  Doris Hillemann; Carsten Haasis; Sönke Andres; Tobias Behn; Katharina Kranzer
Journal:  J Clin Microbiol       Date:  2018-05-25       Impact factor: 5.948

7.  Fluoromycobacteriophages Can Detect Viable Mycobacterium tuberculosis and Determine Phenotypic Rifampicin Resistance in 3-5 Days From Sputum Collection.

Authors:  Liliana Rondón; Estefanía Urdániz; Cecilia Latini; Florencia Payaslian; Mario Matteo; Ezequiel J Sosa; Darío F Do Porto; Adrian G Turjanski; Sergio Nemirovsky; Graham F Hatfull; Susana Poggi; Mariana Piuri
Journal:  Front Microbiol       Date:  2018-07-05       Impact factor: 5.640

8.  Evaluation of the tuberculosis culture color plate test for rapid detection of drug susceptible and drug-resistant Mycobacterium tuberculosis in a resource-limited setting, Addis Ababa, Ethiopia.

Authors:  Biruk Mekonnen; Adane Mihret; Muluwork Getahun; Tsegaye Hailu; Sabeen Sidiki; Holden V Kelley; Julia M Scordo; W Garrett Hunt; Xueliang Pan; Joan-Miquel Balada-Llasat; Wondwossen Gebreyes; Carlton A Evans; Abraham Aseffa; Jordi B Torrelles; Shu-Hua Wang; Tamrat Abebe
Journal:  PLoS One       Date:  2019-05-28       Impact factor: 3.240

9.  Discordance across Phenotypic and Molecular Methods for Drug Susceptibility Testing of Drug-Resistant Mycobacterium tuberculosis Isolates in a Low TB Incidence Country.

Authors:  Suhail Ahmad; Eiman Mokaddas; Noura Al-Mutairi; Hanaa S Eldeen; Shirin Mohammadi
Journal:  PLoS One       Date:  2016-04-20       Impact factor: 3.240

Review 10.  Xpert MTB/RIF assay for the diagnosis of rifampicin resistance in different regions: a meta-analysis.

Authors:  Kaican Zong; Chen Luo; Hui Zhou; Yangzhi Jiang; Shiying Li
Journal:  BMC Microbiol       Date:  2019-08-05       Impact factor: 3.605
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