Literature DB >> 25605579

High-throughput detection of DNA double-strand breaks using image cytometry.

Tyler L Fowler1, Alison M Bailey2, Bryan P Bednarz3, Randall J Kimple4.   

Abstract

Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting DNA double-strand breaks using automated image cytometry assessment of cell average γH2AX immunofluorescence. Our technique provides an expedient, high-throughput, objective, and cost-effective method for γH2AX analysis.

Entities:  

Keywords:  DNA; double-strand breaks; image cytometry; γH2AX

Mesh：

Year:  2015        PMID： 25605579      PMCID： PMC4331074          DOI： 10.2144/000114248

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  6 in total

1.  A novel high-throughput irradiator for in vitro radiation sensitivity bioassays.

Authors:  Tyler L Fowler; Regina K Fulkerson; John A Micka; Randall J Kimple; Bryan P Bednarz
Journal:  Phys Med Biol       Date:  2014-02-28       Impact factor: 3.609

Review 2.  gamma-H2AX as protein biomarker for radiation exposure.

Authors:  Kai Rothkamm; Simon Horn
Journal:  Ann Ist Super Sanita       Date:  2009       Impact factor: 1.663

Review 3.  Gamma-H2AX - a novel biomarker for DNA double-strand breaks.

Authors:  Linda J Kuo; Li-Xi Yang
Journal:  In Vivo       Date:  2008 May-Jun       Impact factor: 2.155

Review 4.  Double strand break repair functions of histone H2AX.

Authors:  Ralph Scully; Anyong Xie
Journal:  Mutat Res       Date:  2013-07-31       Impact factor: 2.433

5.  Histone H2AX phosphorylation: a marker for DNA damage.

Authors:  Arishya Sharma; Kamini Singh; Alexandru Almasan
Journal:  Methods Mol Biol       Date:  2012

6.  DNA-damage foci to detect and characterize DNA repair alterations in children treated for pediatric malignancies.

Authors:  Nadine Schuler; Jan Palm; Mareike Kaiser; Dominik Betten; Rhoikos Furtwängler; Christian Rübe; Norbert Graf; Claudia E Rübe
Journal:  PLoS One       Date:  2014-03-17       Impact factor: 3.240
  6 in total
  4 in total

1.  High-throughput quantitative detection of basal autophagy and autophagic flux using image cytometry.

Authors:  Gopika SenthilKumar; Justin H Skiba; Randall J Kimple
Journal:  Biotechniques       Date:  2019-06-26       Impact factor: 1.993

2.  Multiplexed-Based Assessment of DNA Damage Response to Chemotherapies Using Cell Imaging Cytometry.

Authors:  Nadia Vezzio-Vié; Marie-Alice Kong-Hap; Eve Combès; Augusto Faria Andrade; Maguy Del Rio; Philippe Pasero; Charles Theillet; Céline Gongora; Philippe Pourquier
Journal:  Int J Mol Sci       Date:  2022-05-20       Impact factor: 6.208

3.  AXL Is a Logical Molecular Target in Head and Neck Squamous Cell Carcinoma.

Authors:  Toni M Brand; Mari Iida; Andrew P Stein; Kelsey L Corrigan; Cara M Braverman; John P Coan; Hannah E Pearson; Harsh Bahrar; Tyler L Fowler; Bryan P Bednarz; Sandeep Saha; David Yang; Parkash S Gill; Mark W Lingen; Vassiliki Saloura; Victoria M Villaflor; Ravi Salgia; Randall J Kimple; Deric L Wheeler
Journal:  Clin Cancer Res       Date:  2015-03-12       Impact factor: 12.531

4.  Biological characterization of a novel in vitro cell irradiator.

Authors:  Tyler L Fowler; Michael M Fisher; Alison M Bailey; Bryan P Bednarz; Randall J Kimple
Journal:  PLoS One       Date:  2017-12-12       Impact factor: 3.240
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.