Xiao-Hua Wu1, Yun-Yun Li1, Ping-Ping Zhang1, Kang-Wei Qian1, Jian-Hua Ding2, Gang Hu2, Shi-Jun Weng1, Xiong-Li Yang1, Yong-Mei Zhong1. 1. Institute of Neurobiology, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China. 2. Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu, China.
Abstract
PURPOSE: Retinal dopamine has been long implicated in the signaling pathway regulating eye growth, as evidenced by its reduced levels in myopic eyes in various species. We examined whether and how retinal dopamine levels were changed in a C57BL/6 mouse model of experimental myopia. METHODS: Form-deprivation myopia (FDM) was induced in C57BL/6 mice by wearing monocular occluder for 4 weeks. Refractive errors were measured using an infrared photorefractor. Retinal dopamine/DOPAC and vitreal DOPAC levels were assessed by high-performance liquid chromatography (HPLC). Extracellular dopamine concentrations were examined by Western blot analysis of dopamine transporter (DAT) expression levels. The intactness of retinal dopaminergic system was evaluated by counting tyrosine hydroxylase (TH) immunoreactive cells, measuring the areas occupied by processes of these cells, and quantifying retinal TH expression at both protein and transcription levels. RESULTS: Form-deprivation myopia was successfully induced in C57BL/6 mice with the refractive status of deprived eyes being significantly different from fellow eyes. Unlike most of the previous results obtained in other myopic animal models, however, no significant changes in retinal dopamine, DOPAC, DAT, and vitreal DOPAC levels were detected in deprived eyes, either in the daytime or at night. Furthermore, neither the number of dopaminergic amacrine cells, the area size occupied by the processes of these cells, nor retinal TH expression, were altered in deprived eyes. CONCLUSIONS: The retinal dopamine system remains intact in C57BL/6 mice with FDM, and retinal dopamine levels are not associated with the development of FDM in this mouse strain. Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.
PURPOSE: Retinal dopamine has been long implicated in the signaling pathway regulating eye growth, as evidenced by its reduced levels in myopic eyes in various species. We examined whether and how retinal dopamine levels were changed in a C57BL/6 mouse model of experimental myopia. METHODS: Form-deprivation myopia (FDM) was induced in C57BL/6 mice by wearing monocular occluder for 4 weeks. Refractive errors were measured using an infrared photorefractor. Retinal dopamine/DOPAC and vitreal DOPAC levels were assessed by high-performance liquid chromatography (HPLC). Extracellular dopamine concentrations were examined by Western blot analysis of dopamine transporter (DAT) expression levels. The intactness of retinal dopaminergic system was evaluated by counting tyrosine hydroxylase (TH) immunoreactive cells, measuring the areas occupied by processes of these cells, and quantifying retinal TH expression at both protein and transcription levels. RESULTS: Form-deprivation myopia was successfully induced in C57BL/6 mice with the refractive status of deprived eyes being significantly different from fellow eyes. Unlike most of the previous results obtained in other myopic animal models, however, no significant changes in retinal dopamine, DOPAC, DAT, and vitreal DOPAC levels were detected in deprived eyes, either in the daytime or at night. Furthermore, neither the number of dopaminergic amacrine cells, the area size occupied by the processes of these cells, nor retinal TH expression, were altered in deprived eyes. CONCLUSIONS: The retinal dopamine system remains intact in C57BL/6 mice with FDM, and retinal dopamine levels are not associated with the development of FDM in this mouse strain. Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.
Entities:
Keywords:
C57 mouse; dopamine; form deprivation; myopia; tyrosine hydroxylase
Authors: David Troilo; Earl L Smith; Debora L Nickla; Regan Ashby; Andrei V Tkatchenko; Lisa A Ostrin; Timothy J Gawne; Machelle T Pardue; Jody A Summers; Chea-Su Kee; Falk Schroedl; Siegfried Wahl; Lyndon Jones Journal: Invest Ophthalmol Vis Sci Date: 2019-02-28 Impact factor: 4.799
Authors: Ranjay Chakraborty; Han Na Park; Adam M Hanif; Curran S Sidhu; P Michael Iuvone; Machelle T Pardue Journal: Exp Eye Res Date: 2015-06-10 Impact factor: 3.467
Authors: Ranjay Chakraborty; Victoria Yang; Han Na Park; Erica G Landis; Susov Dhakal; Cara T Motz; Michael A Bergen; P Michael Iuvone; Machelle T Pardue Journal: Exp Eye Res Date: 2018-12-31 Impact factor: 3.467
Authors: Ranjay Chakraborty; Lisa A Ostrin; Debora L Nickla; P Michael Iuvone; Machelle T Pardue; Richard A Stone Journal: Ophthalmic Physiol Opt Date: 2018-05 Impact factor: 3.117
Authors: Michael A Bergen; Han Na Park; Ranjay Chakraborty; Erica G Landis; Curran Sidhu; Li He; P Michael Iuvone; Machelle T Pardue Journal: Invest Ophthalmol Vis Sci Date: 2016-08-01 Impact factor: 4.799