Transport of , , and microspheres in biochar-amended soils with different textures.

The incorporation of biochar into soils has been proposed as a means to sequester carbon from the atmosphere. An added environmental benefit is that biochar has been shown to increase soil retention of agrochemicals, and recent research has indicated that biochar may be effective in increasing soil retention of bacteria. In this study we investigate the transport behavior of O157:H7, serovar Typhimurium, and carboxylated polystyrene microspheres in water-saturated column experiments for two soils (fine sand and sandy loam) amended with 2% poultry litter or pine chip biochars pyrolyzed at 350 and 700°C. Adding poultry litter biochar pyrolyzed at 350°C did not improve soil retention of either bacteria in fine sand and even facilitated their transport in sandy loam. Addition of either biochar pyrolyzed at 700°C generally improved retention of bacteria in fine sand, with the pine chip biochars being more effective in limiting their transport. Results from the column studies and auxiliary batch studies suggest that changes in cell retention after biochar amendments were likely due to changes in bacterial attachment in the column and not to physical straining or changes in survivability. We also found that changes in bacterial hydrophobicity after biochar amendments were generally correlated with changes in bacterial retention. The influence of biochar amendment in increasing retention of both bacteria was generally more pronounced in fine sand and indicates that soil texture affects the transport behavior of bacteria through biochar-amended soils.