Gabriel Cahua-Pablo1, Miguel Cruz2, Oscar Del Moral-Hernández3, Marco A Leyva-Vázquez3, Diana L Antúnez-Ortiz2, José A Cahua-Pablo1, Luz Del Carmen Alarcón-Romero4, Carlos Ortuño-Pineda1, Ma Elena Moreno-Godínez1, Daniel Hernández-Sotelo3, Eugenia Flores-Alfaro5. 1. Clinical and Molecular Epidemiology Laboratory, UA of Chemical and Biological Sciences, Autonomous University of Guerrero, Mexico. 2. Biochemistry Medical Research Unit, Specialties Hospital, Medical Center Century XXI, Mexican Institute of Social Security, México, Distrito Federal. 3. Laboratory of Molecular Biomedicine, UA of Chemical and Biological Sciences, Autonomous University of Guerrero, Mexico. 4. Clinical and Molecular Epidemiology Laboratory, UA of Chemical and Biological Sciences, Autonomous University of Guerrero, Mexico Laboratory of Molecular Biomedicine, UA of Chemical and Biological Sciences, Autonomous University of Guerrero, Mexico. 5. Clinical and Molecular Epidemiology Laboratory, UA of Chemical and Biological Sciences, Autonomous University of Guerrero, Mexico efloresa_2@hotmail.com.
Abstract
INTRODUCTION: Apolipoprotein E (ApoE) 4 isoform has been associated with elevated levels of cholesterol, low-density lipoprotein cholesterol (LDL-C), and triglycerides (TGs), meanwhile several polymorphisms in the LDL receptor (LDLR) gene have been associated with increased levels of total cholesterol and LDL-C. MATERIAL AND METHODS: We studied 400 women from Southwest Mexico. Anthropometric features and biochemical profile were evaluated, and genotyping of single nucleotide polymorphisms rs429358 and rs7412 in the APOE gene and rs688 in the LDLR gene was determined by TaqMan assays. RESULTS: We found significant association between LDL-C (odds ratio [OR] = 3.3, 95% confidence interval [CI]: 1.9-5.7) and marginal association with TG (OR = 1.7, 95% CI: 1.0-2.9) of atherogenic risk in women carriers of the ApoE4 isoform compared to ApoE3. The TT genotype of rs688 in the LDLR gene was not found to be associated with elevated levels of total cholesterol or LDL-C. CONCLUSION: Our results show that carrier women of the ApoE4 isoform are more likely to have elevated levels of LDL-C and therefore increased risk of developing atherosclerosis.
INTRODUCTION:Apolipoprotein E (ApoE) 4 isoform has been associated with elevated levels of cholesterol, low-density lipoprotein cholesterol (LDL-C), and triglycerides (TGs), meanwhile several polymorphisms in the LDL receptor (LDLR) gene have been associated with increased levels of total cholesterol and LDL-C. MATERIAL AND METHODS: We studied 400 women from Southwest Mexico. Anthropometric features and biochemical profile were evaluated, and genotyping of single nucleotide polymorphisms rs429358 and rs7412 in the APOE gene and rs688 in the LDLR gene was determined by TaqMan assays. RESULTS: We found significant association between LDL-C (odds ratio [OR] = 3.3, 95% confidence interval [CI]: 1.9-5.7) and marginal association with TG (OR = 1.7, 95% CI: 1.0-2.9) of atherogenic risk in women carriers of the ApoE4 isoform compared to ApoE3. The TT genotype of rs688 in the LDLR gene was not found to be associated with elevated levels of total cholesterol or LDL-C. CONCLUSION: Our results show that carrier women of the ApoE4 isoform are more likely to have elevated levels of LDL-C and therefore increased risk of developing atherosclerosis.