Proopiomelanocortin (POMC)-related peptides in the brain of the rainbow trout, Salmo gairdneri.

We have investigated the presence of ACTH, alpha-MSH and beta-endorphin, three peptides which derive from the multifunctional precursor protein proopiomelanocortin (POMC) in the brain of the rainbow trout Salmo gairdneri. Using both the indirect immunofluorescence and peroxidase-antiperoxidase techniques, a discrete group of positive cells was identified in the hypothalamus, within the anterior part of the nucleus lateralis tuberis. alpha-MSH-containing neurons represented the most abundant immunoreactive subpopulation. Coexistence of alpha-MSH, ACTH and beta-endorphin was observed in the lateral part of the nucleus. ACTH- and beta-endorphin-containing cells were mainly distributed in the rostral and caudal regions of the nucleus. In the medial portion of the nucleus lateralis tuberis, numerous cells were only stained for alpha-MSH. Moderate to dense plexuses of immunoreactive fibers were observed in the ventral thalamus and the floor of the hypothalamus. Some of these fibers projected towards the pituitary. The concentrations of ACTH, alpha-MSH and beta-endorphin-like immunoreactivities were measured in microdissected brain regions by means of specific radioimmunoassays. Diencephalon, mesencephalon and medulla oblongata extracts gave dilution curves which were parallel to standard curves. The highest concentrations of POMC-derived peptides were found in the diencephalon (alpha-MSH: 4.28 +/- 0.43 ng/mg prot.; ACTH: 1.08 +/- 0.09 ng/mg prot.; beta-endorphin: 1.02 +/- 0.1 ng/mg prot.), while lower concentrations were detected in the mesencephalon, medulla oblongata and telencephalon. The present results demonstrate that various peptides derived from POMC coexist within the same cell bodies of the fish hypothalamus. Taken together, these data suggest that expression and processing of POMC in the fish brain is similar to that occurring in pituitary melanotrophs.