Angela M Moro1, Natália Brucker1, Mariele F Charão1, Elisa Sauer1, Fernando Freitas2, Juliano Durgante2, Guilherme Bubols2, Sarah Campanharo2, Rafael Linden3, Ana P Souza4, Cristina Bonorino4, Rafael Moresco5, Diogo Pilger6, Adriana Gioda7, Sandra Farsky8, Albert Duschl9, Solange C Garcia10. 1. Laboratory of Toxicology (LATOX), Department of Analysis, Pharmacy Faculty, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil; Post-Graduate Programme in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. 2. Laboratory of Toxicology (LATOX), Department of Analysis, Pharmacy Faculty, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. 3. Health Sciences Institute, Feevale University, Novo Hamburgo, RS, Brazil. 4. Laboratory of Cellular and Molecular Immunology, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil. 5. Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, RS, Brazil. 6. Post-Graduate Programme in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. 7. Department of Chemistry of Pontifical Catholic University Rio de Janeiro, Rio de Janeiro, RJ, Brazil. 8. Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil. 9. Department of Molecular Biology, University of Salzburg, Salzburg, Austria. 10. Laboratory of Toxicology (LATOX), Department of Analysis, Pharmacy Faculty, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil; Post-Graduate Programme in Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: solange.garcia@ufrgs.br.
Abstract
INTRODUCTION: Elucidation of effective biomarkers may provide tools for the early detection of biological alterations caused by benzene exposure and may contribute to the reduction of occupational diseases. This study aimed to assess early alterations on hematological and immunological systems of workers exposed to benzene. METHODS: Sixty gasoline station attendants (GSA group) and 28 control subjects were evaluated. Environmental and biological monitoring of benzene exposure was performed in blood and urine. The potential effect biomarkers evaluated were δ-aminolevulinate dehydratase (ALA-D) activity, CD80 and CD86 expression in lymphocytes and monocytes, and serum interleukin-8 (IL-8). The influence of confounding factors and toluene co-exposure were considered. RESULTS: Although exposures were below ACGIH (American Conference of Governmental Industrial Hygienists) limits, reduced ALA-D activity, decreased CD80 and CD86 expression in monocytes and increased IL-8 levels were found in the GSA group compared to the control subjects. Furthermore, according to multiple linear regression analysis, benzene exposure was associated to a decrease in CD80 and CD86 expression in monocytes. CONCLUSIONS: These findings suggest, for the first time, a potential effect of benzene exposure on ALA-D activity, CD80 and CD86 expression, IL-8 levels, which could be suggested as potential markers for the early detection of benzene-induced alterations.
INTRODUCTION: Elucidation of effective biomarkers may provide tools for the early detection of biological alterations caused by benzene exposure and may contribute to the reduction of occupational diseases. This study aimed to assess early alterations on hematological and immunological systems of workers exposed to benzene. METHODS: Sixty gasoline station attendants (GSA group) and 28 control subjects were evaluated. Environmental and biological monitoring of benzene exposure was performed in blood and urine. The potential effect biomarkers evaluated were δ-aminolevulinate dehydratase (ALA-D) activity, CD80 and CD86 expression in lymphocytes and monocytes, and serum interleukin-8 (IL-8). The influence of confounding factors and toluene co-exposure were considered. RESULTS: Although exposures were below ACGIH (American Conference of Governmental Industrial Hygienists) limits, reduced ALA-D activity, decreased CD80 and CD86 expression in monocytes and increased IL-8 levels were found in the GSA group compared to the control subjects. Furthermore, according to multiple linear regression analysis, benzene exposure was associated to a decrease in CD80 and CD86 expression in monocytes. CONCLUSIONS: These findings suggest, for the first time, a potential effect of benzene exposure on ALA-D activity, CD80 and CD86 expression, IL-8 levels, which could be suggested as potential markers for the early detection of benzene-induced alterations.
Authors: Mohamed F Abou Elazab; Asmaa E A Elbaiomy; Mohamed S Ahmed; Khalaf F Alsharif; Naief Dahran; Ehab Kotb Elmahallawy; Abdallah A Mokhbatly Journal: Front Vet Sci Date: 2022-06-22
Authors: Courtney Schiffman; Cliona M McHale; Alan E Hubbard; Luoping Zhang; Reuben Thomas; Roel Vermeulen; Guilan Li; Min Shen; Stephen M Rappaport; Songnian Yin; Qing Lan; Martyn T Smith; Nathaniel Rothman Journal: PLoS One Date: 2018-10-09 Impact factor: 3.240