AIM: A new paromomycin micellar nanogel based on poloxamer 407 was developed. MATERIALS & METHODS: In vitro release and ex vivo permeation/retention studies were conducted. In vivo tolerance was assayed by transepidermal water loss. Ex vivo cytotoxicity on RAW and VERO cells and antileishmanial activity on Leishmania promastigotes was tested. RESULTS: The particle size was 9.19 nm (99% loading efficiency) exhibiting Newtonian behavior at 4°C and was pseudoplastic at 25 and 40°C. Drug release followed a Weibull model and the drug remaining in the skin was 31.652 µg/g/cm(2). In vivo tolerance achieved excellent results with negligible cellular toxicity and the best antileishmanial efficiency. CONCLUSION: The nanogel provided controlled, effective and safe delivery of paromomycin for the treatment of cutaneous leishmaniasis.
AIM: A new paromomycin micellar nanogel based on poloxamer 407 was developed. MATERIALS & METHODS: In vitro release and ex vivo permeation/retention studies were conducted. In vivo tolerance was assayed by transepidermal water loss. Ex vivo cytotoxicity on RAW and VERO cells and antileishmanial activity on Leishmania promastigotes was tested. RESULTS: The particle size was 9.19 nm (99% loading efficiency) exhibiting Newtonian behavior at 4°C and was pseudoplastic at 25 and 40°C. Drug release followed a Weibull model and the drug remaining in the skin was 31.652 µg/g/cm(2). In vivo tolerance achieved excellent results with negligible cellular toxicity and the best antileishmanial efficiency. CONCLUSION: The nanogel provided controlled, effective and safe delivery of paromomycin for the treatment of cutaneous leishmaniasis.
Authors: Mireia Mallandrich; Francisco Fernández-Campos; Beatriz Clares; Lyda Halbaut; Cristina Alonso; Luisa Coderch; Maria L Garduño-Ramírez; Berenice Andrade; Alfonso Del Pozo; Majella E Lane; Ana C Calpena Journal: Pharm Res Date: 2017-05-24 Impact factor: 4.200
Authors: Letícia Martins Dos Reis Lage; José Mário Barichello; Daniela Pagliara Lage; Débora Vasconcelos Costa Mendonça; Ana Maria Ravena Severino Carvalho; Marcella Rezende Rodrigues; Daniel Menezes-Souza; Bruno Mendes Roatt; Ricardo José Alves; Carlos Alberto Pereira Tavares; Eduardo Antonio Ferraz Coelho; Mariana Costa Duarte Journal: Parasitol Res Date: 2016-07-01 Impact factor: 2.289
Authors: Natascia Bruni; Barbara Stella; Leonardo Giraudo; Carlo Della Pepa; Daniela Gastaldi; Franco Dosio Journal: Int J Nanomedicine Date: 2017-07-26
Authors: Noelia Pérez-González; Nuria Bozal-de Febrer; Ana C Calpena-Campmany; Anna Nardi-Ricart; María J Rodríguez-Lagunas; José A Morales-Molina; José L Soriano-Ruiz; Francisco Fernández-Campos; Beatriz Clares-Naveros Journal: Gels Date: 2021-12-12