Esben S Lauritzen1, Thomas Voss2, Ulla Kampmann3, Annette Mengel2, Mikkel H Vendelbo2, Jens O L Jørgensen3, Niels Møller3, Esben T Vestergaard2. 1. Medical Research LaboratoryAarhus University, Nørrebrogade 44, Building 3B, 8000 Aarhus C, DenmarkDepartment of Endocrinology and Internal MedicineAarhus University Hospital, Nørrebrogade 44, Building 2A, 8000 Aarhus C, Denmark esben.lauritzen@clin.au.dk. 2. Medical Research LaboratoryAarhus University, Nørrebrogade 44, Building 3B, 8000 Aarhus C, DenmarkDepartment of Endocrinology and Internal MedicineAarhus University Hospital, Nørrebrogade 44, Building 2A, 8000 Aarhus C, Denmark. 3. Medical Research LaboratoryAarhus University, Nørrebrogade 44, Building 3B, 8000 Aarhus C, DenmarkDepartment of Endocrinology and Internal MedicineAarhus University Hospital, Nørrebrogade 44, Building 2A, 8000 Aarhus C, Denmark Medical Research LaboratoryAarhus University, Nørrebrogade 44, Building 3B, 8000 Aarhus C, DenmarkDepartment of Endocrinology and Internal MedicineAarhus University Hospital, Nørrebrogade 44, Building 2A, 8000 Aarhus C, Denmark.
Abstract
OBJECTIVE: Ghrelin has glucoregulatory and orexigenic actions, but its role in acute hypoglycemia remains uncertain. We aimed to investigate circulating levels of acylghrelin (AG) and unacylated ghrelin (UAG) in response to hyperinsulinemia and to hypoglycemia. DESIGN: A randomized, single-blind, placebo-controlled crossover study including 3 study days was performed at a university hospital clinical research center. METHODS:Nine healthy men completed 3 study days: i) saline control (CTR), ii) hyperinsulinemic euglycemia (HE) (bolus insulin 0.1 IE/kg i.v. and glucose 20% i.v. for 105 min, plasma glucose ≈5 mmol/l), and iii) hyperinsulinemic hypoglycemia (HH) (bolus insulin 0.1 IE/kg i.v.). RESULTS:HH and HE suppressed AG concentrations at t=45-60 min as compared with CTR (P<0.05). At t=90 min, a rebound increase in AG was observed in response to HH as compared with both HE and CTR (P<0.05). UAG also decreased during HH and HE at t=45 min (P<0.05), whereas the AG-to-UAG ratio remained unaffected. CONCLUSIONS: This study demonstrates that AG and UAG are directly suppressed by hyperinsulinemia and that AG concentrations increase after a latency of ≈1 h in response to hypoglycemia, suggesting a potential counterregulatory role of AG.
RCT Entities:
OBJECTIVE:Ghrelin has glucoregulatory and orexigenic actions, but its role in acute hypoglycemia remains uncertain. We aimed to investigate circulating levels of acylghrelin (AG) and unacylated ghrelin (UAG) in response to hyperinsulinemia and to hypoglycemia. DESIGN: A randomized, single-blind, placebo-controlled crossover study including 3 study days was performed at a university hospital clinical research center. METHODS: Nine healthy men completed 3 study days: i) saline control (CTR), ii) hyperinsulinemic euglycemia (HE) (bolus insulin 0.1 IE/kg i.v. and glucose 20% i.v. for 105 min, plasma glucose ≈5 mmol/l), and iii) hyperinsulinemic hypoglycemia (HH) (bolus insulin 0.1 IE/kg i.v.). RESULTS:HH and HE suppressed AG concentrations at t=45-60 min as compared with CTR (P<0.05). At t=90 min, a rebound increase in AG was observed in response to HH as compared with both HE and CTR (P<0.05). UAG also decreased during HH and HE at t=45 min (P<0.05), whereas the AG-to-UAG ratio remained unaffected. CONCLUSIONS: This study demonstrates that AG and UAG are directly suppressed by hyperinsulinemia and that AG concentrations increase after a latency of ≈1 h in response to hypoglycemia, suggesting a potential counterregulatory role of AG.
Authors: Thomas S Voss; Mikkel H Vendelbo; Ulla Kampmann; Steen B Pedersen; Thomas S Nielsen; Mogens Johannsen; Mads V Svart; Niels Jessen; Niels Møller Journal: Diabetologia Date: 2016-10-12 Impact factor: 10.122
Authors: Kripa Shankar; Deepali Gupta; Bharath K Mani; Brianna G Findley; Sherri Osborne-Lawrence; Nathan P Metzger; Chen Liu; Eric D Berglund; Jeffrey M Zigman Journal: Front Endocrinol (Lausanne) Date: 2020-09-11 Impact factor: 5.555