The emission of stress-induced 22-kHz calls in female rats is independent of testosterone levels.

Although emission of ultrasonic calls in rats induced by stress, referred to as "22-kHz calls," is dependent on circulating testosterone levels in males, it is still unknown whether the same testosterone-based regulation is applicable to female rats. In this study, we investigated the sex difference in the emission of air-puff-induced 22-kHz calls in rats on the basis of the hypothesis that female rats would emit fewer 22-kHz calls, and assessed whether male-like circulating testosterone levels can also influence the emission of 22-kHz calls in females. The experimental results showed that female rats emit significantly fewer 22-kHz calls than male rats. However, male-like circulating testosterone levels have little effect on the emission of 22-kHz calls in females. Hence, it is suggested that there is a sex difference in stress-induced 22-kHz calls in rats and, in contrast to male rats, the emission of 22-kHz calls in female rats may be independent of circulating testosterone levels, as tested in response to air-puff stimulation.