Literature DB >> 25596381

Isolation of isochlorogenic acid isomers in flower buds of Lonicera japonica by high-speed counter-current chromatography and preparative high performance liquid chromatography.

Wei Guo1, Liang Wang1, Yan Gao1, Bonian Zhao1, Daijie Wang2, Wenjuan Duan2, Zongyuan Yu3.   

Abstract

An efficient method was established by HSCCC combined with prep-HPLC for separating isochlorogenic acid isomers from flower buds of Lonicera japonica. The partially purified sample from the methanol extract of flower buds of L. japonica by silica gel column was separated by HSCCC to result in a fraction containing two isochlorogenic acid isomers. The fraction was further isolated by prep-HPLC to yield isochlorogenic acid A and isochlorogenic acid C with purities of 98% and 96%, and the total recoveries at 80.1% and 79.8%, respectively. The chemical structures of isochlorogenic acid isomers were confirmed by electrospray ionization mass spectrometry (ESI-MS) and (1)H nuclear magnetic resonance (NMR).
Copyright © 2014 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Flower buds of Lonicera japonica; High-speed counter-current chromatography; Isochlorogenic acid A; Isochlorogenic acid C; Preparative high performance liquid chromatography

Mesh：

Substances：

Year:  2015        PMID： 25596381     DOI： 10.1016/j.jchromb.2014.12.020

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  7 in total

1.  Preparative separation of seven phenolic acids from Xanthii Fructus using pH-zone-refining counter-current chromatography combined with semi-preparative high performance liquid chromatography.

Authors:  Tianyu Ma; Lihua Xu; Xinming Wang; Jia Li; Lanping Guo; Xiao Wang
Journal:  RSC Adv       Date:  2019-11-11       Impact factor: 4.036

2.  Isochlorogenic acid C prevents enterovirus 71 infection via modulating redox homeostasis of glutathione.

Authors:  Zeyu Cao; Yue Ding; Liang Cao; Gang Ding; Zhenzhong Wang; Wei Xiao
Journal:  Sci Rep       Date:  2017-11-24       Impact factor: 4.379

3.  Identification of Transient Receptor Potential Vanilloid 3 Antagonists from Achillea alpina L. and Separation by Liquid-Liquid-Refining Extraction and High-Speed Counter-Current Chromatography.

Authors:  Shi-Wei Sun; Rong-Rong Wang; Xiao-Ying Sun; Jia-He Fan; Hang Qi; Yang Liu; Guo-Qing Qin; Wei Wang
Journal:  Molecules       Date:  2020-04-26       Impact factor: 4.411

4.  HSCCC Separation of the Two Iridoid Glycosides and Three Phenolic Compounds from Veronica ciliata and Their in Vitro Antioxidant and Anti-Hepatocarcinoma Activities.

Authors:  Qiuxia Lu; Yiran Sun; Yueyue Shu; Shancai Tan; Li Yin; Yiran Guo; Lin Tang
Journal:  Molecules       Date:  2016-09-15       Impact factor: 4.411

5.  An Efficient Method for the Preparative Isolation and Purification of Flavonoid Glycosides and Caffeoylquinic Acid Derivatives from Leaves of Lonicera japonica Thunb. Using High Speed Counter-Current Chromatography (HSCCC) and Prep-HPLC Guided by DPPH-HPLC Experiments.

Authors:  Daijie Wang; Ning Du; Lei Wen; Heng Zhu; Feng Liu; Xiao Wang; Jinhua Du; Shengbo Li
Journal:  Molecules       Date:  2017-02-02       Impact factor: 4.411

6.  Healthspan Improvements in Caenorhabditis elegans with Traditional Chinese Herbal Tea.

Authors:  Chunxiu Lin; Xiaoying Zhang; Chuting Zhuang; Yugui Lin; Yong Cao; Yunjiao Chen
Journal:  Oxid Med Cell Longev       Date:  2020-12-16       Impact factor: 6.543

7.  Isolation of Two Isochlorogenic Acid Isomers from Phenolic Rich Fraction of Artemisia turanica Krasch.

Authors:  Fahimeh Moradi-Afrapoli; Ghazal Saremi; Sajjad Nasseri; Seyed Ahmad Emami; Mahdi Mojarrab
Journal:  Iran J Pharm Res       Date:  2020       Impact factor: 1.696
  7 in total

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