Radhia M'kacher1, Elie El Maalouf2, Georgia Terzoudi3, Michelle Ricoul1, Leonhard Heidingsfelder4, Ionna Karachristou3, Eric Laplagne5, William M Hempel1, Bruno Colicchio6, Alain Dieterlen6, Gabriel Pantelias3, Laure Sabatier7. 1. Laboratoire de Radiobiologie et Oncologie, Commissariat à l'Energie Atomique, Fontenay-aux-Roses, France. 2. Laboratoire de Radiobiologie et Oncologie, Commissariat à l'Energie Atomique, Fontenay-aux-Roses, France; Laboratoire Modélisation Intelligence Processus Systèmes (MIPS)-Groupe TIIM3D, Université de Haute-Alsace, Mulhouse, France. 3. Laboratory of Radiobiology & Biodosimetry, National Center for Scientific Research Demokritos, Athens, Greece. 4. MetaSystems, Altlussheim, Germany. 5. Pole Concept, Paris, France. 6. Laboratoire Modélisation Intelligence Processus Systèmes (MIPS)-Groupe TIIM3D, Université de Haute-Alsace, Mulhouse, France. 7. Laboratoire de Radiobiologie et Oncologie, Commissariat à l'Energie Atomique, Fontenay-aux-Roses, France. Electronic address: laure.sabatier@cea.fr.
Abstract
PURPOSE: To combine telomere and centromere (TC) staining of premature chromosome condensation (PCC) fusions to identify dicentrics, centric rings, and acentric chromosomes, making possible the realization of a dose-response curve and automation of the process. METHODS AND MATERIALS: Blood samples from healthy donors were exposed to (60)Co irradiation at varying doses up to 8 Gy, followed by a repair period of 8 hours. Premature chromosome condensation fusions were carried out, and TC staining using peptide nucleic acid probes was performed. Chromosomal aberration (CA) scoring was carried out manually and automatically using PCC-TCScore software, developed in our laboratory. RESULTS: We successfully optimized the hybridization conditions and image capture parameters, to increase the sensitivity and effectiveness of CA scoring. Dicentrics, centric rings, and acentric chromosomes were rapidly and accurately detected, leading to a linear-quadratic dose-response curve by manual scoring at up to 8 Gy. Using PCC-TCScore software for automatic scoring, we were able to detect 95% of dicentrics and centric rings. CONCLUSION: The introduction of TC staining to the PCC fusion technique has made possible the rapid scoring of unstable CAs, including dicentrics, with a level of accuracy and ease not previously possible. This new approach can be used for biological dosimetry in radiation emergency medicine, where the rapid and accurate detection of dicentrics is a high priority using automated scoring. Because there is no culture time, this new approach can also be used for the follow-up of patients treated by genotoxic therapy, creating the possibility to perform the estimation of induced chromosomal aberrations immediately after the blood draw.
PURPOSE: To combine telomere and centromere (TC) staining of premature chromosome condensation (PCC) fusions to identify dicentrics, centric rings, and acentric chromosomes, making possible the realization of a dose-response curve and automation of the process. METHODS AND MATERIALS: Blood samples from healthy donors were exposed to (60)Co irradiation at varying doses up to 8 Gy, followed by a repair period of 8 hours. Premature chromosome condensation fusions were carried out, and TC staining using peptide nucleic acid probes was performed. Chromosomal aberration (CA) scoring was carried out manually and automatically using PCC-TCScore software, developed in our laboratory. RESULTS: We successfully optimized the hybridization conditions and image capture parameters, to increase the sensitivity and effectiveness of CA scoring. Dicentrics, centric rings, and acentric chromosomes were rapidly and accurately detected, leading to a linear-quadratic dose-response curve by manual scoring at up to 8 Gy. Using PCC-TCScore software for automatic scoring, we were able to detect 95% of dicentrics and centric rings. CONCLUSION: The introduction of TC staining to the PCC fusion technique has made possible the rapid scoring of unstable CAs, including dicentrics, with a level of accuracy and ease not previously possible. This new approach can be used for biological dosimetry in radiation emergency medicine, where the rapid and accurate detection of dicentrics is a high priority using automated scoring. Because there is no culture time, this new approach can also be used for the follow-up of patients treated by genotoxic therapy, creating the possibility to perform the estimation of induced chromosomal aberrations immediately after the blood draw.
Authors: M A Suresh Kumar; Evagelia C Laiakis; Shanaz A Ghandhi; Shad R Morton; Albert J Fornace; Sally A Amundson Journal: Radiat Res Date: 2018-05-10 Impact factor: 2.841
Authors: Paula Carpintero-Fernandez; Marta Varela-Eirin; Alessandra Lacetera; Raquel Gago-Fuentes; Eduardo Fonseca; Sonsoles Martin-Santamaria; Maria D Mayan Journal: Biomolecules Date: 2020-04-21
Authors: Terri L Ryan; Antonio G Pantelias; Georgia I Terzoudi; Gabriel E Pantelias; Adayabalam S Balajee Journal: PLoS One Date: 2019-05-06 Impact factor: 3.240