BACKGROUND: Recurrent spontaneous abortion impacts almost 1% of couples. The sera from women with unexplained recurrent spontaneous abortion (URSA) have toxic effects on embryos that grow in the uterus. Therefore, the abnormal condition of the uterus may also affect sperm qualities. OBJECTIVE: The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility. MATERIALS AND METHODS: Sera of 20 women with URSA history and sera from 20 women with at least two healthy children were added to the sperms samples from 20 healthy men for 2 hours. The sperm motility was assessed after incubation with sera. The samples were stained with Tdt mediated dUTP nick end labeling (TUNEL) assay for DNA fragmentation. The samples were analyzed with flow cytometry and the percentage of the TUNEL positive sperms were calculated. The data were analyzed by t-test. RESULTS: The incubation of the sperm samples in sera with URSA lead to a decrease in the percentage of the motile sperm from 55% in control to 41% in the treated group, significantly (p=0.038). The percentage of the sperm with abnormal fragmented DNA increased after incubation with URSA (26.6%) compare to the control (21.2%); however, it was not significant. CONCLUSION: It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation. However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility.
BACKGROUND: Recurrent spontaneous abortion impacts almost 1% of couples. The sera from women with unexplained recurrent spontaneous abortion (URSA) have toxic effects on embryos that grow in the uterus. Therefore, the abnormal condition of the uterus may also affect sperm qualities. OBJECTIVE: The objectives of this study were to search if these sera could induce DNA denaturation in sperm nuclei and also it could reduce sperm motility. MATERIALS AND METHODS: Sera of 20 women with URSA history and sera from 20 women with at least two healthy children were added to the sperms samples from 20 healthy men for 2 hours. The sperm motility was assessed after incubation with sera. The samples were stained with Tdt mediated dUTP nick end labeling (TUNEL) assay for DNA fragmentation. The samples were analyzed with flow cytometry and the percentage of the TUNEL positive sperms were calculated. The data were analyzed by t-test. RESULTS: The incubation of the sperm samples in sera with URSA lead to a decrease in the percentage of the motile sperm from 55% in control to 41% in the treated group, significantly (p=0.038). The percentage of the sperm with abnormal fragmented DNA increased after incubation with URSA (26.6%) compare to the control (21.2%); however, it was not significant. CONCLUSION: It seems that sera from URSA patients could not induce a significant increase in the percentage of the sperms with nuclei contain DNA fragmentation. However, the sera of women with URSA could affect the fertility rate by reduction of the sperm motility.
Authors: Pikee Saxena; M M Misro; Somnath Roy; K Chopra; D Sinha; Deoki Nandan; Shubha Sagar Trivedi Journal: Indian J Physiol Pharmacol Date: 2008 Jul-Sep
Authors: D A Ferrari; P A Gilles; N W Klein; D Nadler; B S Weeks; C J Lammi-Keefe; R E Hillman; S W Carey; Y K Ying; D Maier Journal: Am J Obstet Gynecol Date: 1994-01 Impact factor: 8.661
Authors: Raúl Fernández-Gonzalez; Pedro Nuno Moreira; Miriam Pérez-Crespo; Manuel Sánchez-Martín; Miguel Angel Ramirez; Eva Pericuesta; Ainhoa Bilbao; Pablo Bermejo-Alvarez; Juan de Dios Hourcade; Fernando Rodriguez de Fonseca; Alfonso Gutiérrez-Adán Journal: Biol Reprod Date: 2008-01-16 Impact factor: 4.285