H Liu1, Y Li2, J Zhang3, M Rao4, H Liang5, G Liu6. 1. Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, Guangdong 511400, People's Republic of China. Electronic address: lht001018aaa@163.com. 2. Department of Urology, The First Affiliated Hospital, Jinan University, Huangpu Avenue West 601, Guangzhou, Guangdong 510630, People's Republic of China. Electronic address: 6649212@qq.com. 3. Department of Pathology, Guangdong Women and Children Hospital, Guangzhou, Guangdong 511400, People's Republic of China. Electronic address: superchina2000sox@mail.com. 4. Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, Guangdong 511400, People's Republic of China. Electronic address: 1317540757@qq.com. 5. Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, Guangdong 511400, People's Republic of China. Electronic address: lhy2008@126.com. 6. Department of Obstetrics and Gynecology, Guangdong Women and Children Hospital, Guangzhou, Guangdong 511400, People's Republic of China. Electronic address: hnliuguocheng@126.com.
Abstract
INTRODUCTION: Pre-eclampsia (PE) is characterized by failed remodeling of maternal vessels perfusing the placenta. Blood vessels and lymphatic system are involved in vessel remodeling and flow homeostasis in the uterus during pregnancy. This study aims to investigate the involvement of angiogenesis and lymphangiogenesis in PE. METHODS: Placental and decidual tissues were obtained from pregnancies with PE (n = 90), including PE cases with decidual vasculopathy (DV) (n = 52) and without DV (n = 38), and healthy pregnancies (control, n = 20). The clinical characteristics of these groups were analyzed. The expression levels of VEGF1, CD34, PROX-1, VEGFR3, and CD31 in the placenta and decidua were detected through immunohistochemistry, reverse-transcription polymerase chain reaction, and Western blot. RESULTS: The lymphangiogenic markers PROX-1 and VEGFR3 were negatively expressed in the placenta but positively expressed in the decidua. The expression levels of the angiogenic markers VEGF1 and CD34 and the panendothelial marker CD31 were significantly lower in the placenta and decidua of the PE group than in those of the control group. The expression levels of VEGF1, CD34, and CD31 were significantly lower in the placenta and decidua with DV than in those without DV. Furthermore, the expression trends of PROX-1 and VEGFR3 was similar to those of VEGF1, CD34, and CD31 among the groups. CONCLUSIONS: Lymphangiogenesis occurred in the decidua but not in the placenta. Impaired angiogenesis and lymphangiogenesis were associated with PE, particularly in the presence of DV.
INTRODUCTION: Pre-eclampsia (PE) is characterized by failed remodeling of maternal vessels perfusing the placenta. Blood vessels and lymphatic system are involved in vessel remodeling and flow homeostasis in the uterus during pregnancy. This study aims to investigate the involvement of angiogenesis and lymphangiogenesis in PE. METHODS: Placental and decidual tissues were obtained from pregnancies with PE (n = 90), including PE cases with decidual vasculopathy (DV) (n = 52) and without DV (n = 38), and healthy pregnancies (control, n = 20). The clinical characteristics of these groups were analyzed. The expression levels of VEGF1, CD34, PROX-1, VEGFR3, and CD31 in the placenta and decidua were detected through immunohistochemistry, reverse-transcription polymerase chain reaction, and Western blot. RESULTS: The lymphangiogenic markers PROX-1 and VEGFR3 were negatively expressed in the placenta but positively expressed in the decidua. The expression levels of the angiogenic markers VEGF1 and CD34 and the panendothelial marker CD31 were significantly lower in the placenta and decidua of the PE group than in those of the control group. The expression levels of VEGF1, CD34, and CD31 were significantly lower in the placenta and decidua with DV than in those without DV. Furthermore, the expression trends of PROX-1 and VEGFR3 was similar to those of VEGF1, CD34, and CD31 among the groups. CONCLUSIONS: Lymphangiogenesis occurred in the decidua but not in the placenta. Impaired angiogenesis and lymphangiogenesis were associated with PE, particularly in the presence of DV.
Authors: Akriti S Sahay; Anjali T Jadhav; Deepali P Sundrani; Girija N Wagh; Savita S Mehendale; Preeti Chavan-Gautam; Sadhana R Joshi Journal: Mol Cell Biochem Date: 2017-08-02 Impact factor: 3.396
Authors: Miguel A Ortega; Miguel A Saez; Oscar Fraile-Martínez; Ángel Asúnsolo; Leonel Pekarek; Coral Bravo; Santiago Coca; Felipe Sainz; Melchor Álvarez- Mon; Julia Buján; Natalio García-Honduvilla Journal: Int J Mol Sci Date: 2020-04-03 Impact factor: 5.923
Authors: Miguel A Ortega; Oscar Fraile-Martínez; Miguel A Saez; Miguel A Álvarez-Mon; Ana M Gómez-Lahoz; Coral Bravo; Juan A De León Luis; Felipe Sainz; Santiago Coca; Ángel Asúnsolo; Jorge Monserrat; Luis G Guijarro; Melchor Álvarez-Mon; Julia Bujan; Natalio García-Honduvilla Journal: Int J Med Sci Date: 2021-05-27 Impact factor: 3.738