Padma R1, Annaji Sreedhara2, Indeevar P3, Indranil Sarkar4, Chetan S Kumar5. 1. Head of the Department, Department of Periodontics, Coorg Institute of Dental Sciences , Virajpet, Karnataka, India . 2. Reader, Department of Periodontics, Coorg Institute of Dental Sciences , Virajpet, Karnataka, India . 3. Senior Lecturer, Department of Periodontics, St. Joseph Dental College , Eluru, Andhra Pradesh, India . 4. Post Graduate Student, Department of Periodontics, Coorg Institute of Dental Sciences , Virajpet, Karnataka, India . 5. Reader, Department of Orthodontics, St. Joseph Dental College , Eluru, Andhra Pradesh, India .
Abstract
INTRODUCTION: Of the various cytokines and growth factors regulating angiogenesis, the most potent agent acting on vascular endothelium is vascular endothelial growth factor(VEGF). The present study aims to access VEGF concentration in periodontal diseases and the effect of periodontal therapy on its concentration in gingival crevicular fluid (GCF). MATERIALS AND METHODS: Ninety five subjects (47 females and 48 males) 25- 47 y of age were divided into healthy (group-1), gingivitis (group-2), and periodontitis (group-3). GCF samples were collected using micro-capillary pipettes & were transferred immediately to plastic vials and stored at -70˚C until the time of the assay. The concentration of VEGF was determined using commercially available ELISA kit. RESULTS: The mean VEGF concentration was highest in periodontitis patients (Group 3) (88.08±8.04pg/ml), with lowest in healthy patients (Group 1). VEGF levels reduced significantly after therapy in Groups 2 and 3. CONCLUSION: VEGF levels in GCF had a significant correlation with both periodontal disease progression and healing after therapy.
INTRODUCTION: Of the various cytokines and growth factors regulating angiogenesis, the most potent agent acting on vascular endothelium is vascular endothelial growth factor(VEGF). The present study aims to access VEGF concentration in periodontal diseases and the effect of periodontal therapy on its concentration in gingival crevicular fluid (GCF). MATERIALS AND METHODS: Ninety five subjects (47 females and 48 males) 25- 47 y of age were divided into healthy (group-1), gingivitis (group-2), and periodontitis (group-3). GCF samples were collected using micro-capillary pipettes & were transferred immediately to plastic vials and stored at -70˚C until the time of the assay. The concentration of VEGF was determined using commercially available ELISA kit. RESULTS: The mean VEGF concentration was highest in periodontitispatients (Group 3) (88.08±8.04pg/ml), with lowest in healthy patients (Group 1). VEGF levels reduced significantly after therapy in Groups 2 and 3. CONCLUSION:VEGF levels in GCF had a significant correlation with both periodontal disease progression and healing after therapy.
Authors: Lei Cao; Xiangyang Jiao; David S Zuzga; Yuhong Liu; Dahna M Fong; Deborah Young; Matthew J During Journal: Nat Genet Date: 2004-07-18 Impact factor: 38.330
Authors: Federica Romano; Loretta Bongiovanni; Laura Bianco; Federica Di Scipio; Zhiqian Yang; Andrea Elio Sprio; Giovanni Nicolao Berta; Mario Aimetti Journal: Clin Oral Investig Date: 2017-09-16 Impact factor: 3.573
Authors: T Rakmanee; E Calciolari; I Olsen; U Darbar; G S Griffiths; A Petrie; Nikolaos Donos Journal: Clin Oral Investig Date: 2018-11-29 Impact factor: 3.573