Carine Nguemeni1, Mariana Gomez-Smith1, Matthew S Jeffers1, Clarissa Pedrini Schuch2, Dale Corbett3. 1. Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada; Canadian Partnership for Stroke Recovery, University of Ottawa, Ottawa, ON, Canada. 2. Universidade Federal do Rio Grande do Sul, Brazil. 3. Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada; Faculty of Medicine, Memorial University of Newfoundland, St. John's, NL, Canada; Faculty of Medicine, University of Toronto, Toronto, ON, Canada; Canadian Partnership for Stroke Recovery, University of Ottawa, Ottawa, ON, Canada. Electronic address: dcorbett@uottawa.ca.
Abstract
BACKGROUND: Endothelin-1 (ET-1) induced focal ischemia is increasingly being used as a preclinical model of stroke. Here, we described for the first time, the time course of neuronal death and infarct evolution during the first 7 days following ischemia. NEW METHOD: We used hematoxylin and eosin (H&E) staining to evaluate infarct progression and Fluoro-Jade C (FJC) to quantify neuronal degeneration at 24, 48, 72h and 7 days after ET-1 injection to the forelimb motor cortex in Sprague-Dawley rats. RESULTS: We found that infarct volume and neuronal degeneration are maximal at 24h post-stroke. Neuronal degeneration is also significantly reduced within 7 days of stroke induction. COMPARISON WITH EXISTING METHOD: This study is the first to provide a direct evaluation of both infarct volume evolution and neuronal death time course following ET-1 induced focal ischemia in the forelimb motor cortex. CONCLUSION: This study describes the short-term time course of neuronal death and brain injury in the ET-1 stroke model, which provides a significant reference when determining the appropriate time to commence neuroprotective or recovery promoting strategies.
BACKGROUND:Endothelin-1 (ET-1) induced focal ischemia is increasingly being used as a preclinical model of stroke. Here, we described for the first time, the time course of neuronal death and infarct evolution during the first 7 days following ischemia. NEW METHOD: We used hematoxylin and eosin (H&E) staining to evaluate infarct progression and Fluoro-Jade C (FJC) to quantify neuronal degeneration at 24, 48, 72h and 7 days after ET-1 injection to the forelimb motor cortex in Sprague-Dawley rats. RESULTS: We found that infarct volume and neuronal degeneration are maximal at 24h post-stroke. Neuronal degeneration is also significantly reduced within 7 days of stroke induction. COMPARISON WITH EXISTING METHOD: This study is the first to provide a direct evaluation of both infarct volume evolution and neuronal death time course following ET-1 induced focal ischemia in the forelimb motor cortex. CONCLUSION: This study describes the short-term time course of neuronal death and brain injury in the ET-1stroke model, which provides a significant reference when determining the appropriate time to commence neuroprotective or recovery promoting strategies.
Authors: Walther A Carvalho; Carlomagno P Bahia; Jéssica C Teixeira; Walace Gomes-Leal; Antonio Pereira Journal: Front Neuroanat Date: 2017-10-13 Impact factor: 3.856