Au nanoparticles-3D graphene hydrogel nanocomposite to boost synergistically in situ detection sensitivity toward cell-released nitric oxide.

In situ detection of nitric oxide (NO) released from living cells has become very important in studies of some critical physiological and pathological processes, but it is still very challenging due to the low concentration and fast decay of NO. A nanocomposite of Au nanoparticles deposited on three-dimensional graphene hydrogel (Au NPs-3DGH) was prepared through a facile one-step approach by in situ reduction of Au(3+) on 3DGH to build a unique sensing film for a strong synergistic effect, in which the highly porous 3DGH offers a large surface area while Au NPs uniformly deposited on 3DGH efficiently catalyze the electrochemical oxidation of NO for sensitive detection of NO with excellent selectivity, fast response, and low detection limit. The sensor was further used to in situ detect NO released from living cells under drug stimulation, showing significant difference between normal and tumor cells under drug stimulation.