| Literature DB >> 25579596 |
Soraya Ait-Bara1, Caroline Clerté, Emmanuel Margeat.
Abstract
Single-molecule Förster Resonance Energy Transfer (smFRET) is a useful technique to probe conformational changes within bio-macromolecules. Here, we introduce how to perform smFRET measurements in solution to investigate RNA remodeling and RNA-protein interactions. In particular, we focus on how the close-to-open transition of an antiterminator hairpin is influenced by the binding of the antitermination protein and the competition by oligonucleotides.Entities:
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Year: 2015 PMID： 25579596 DOI： 10.1007/978-1-4939-2214-7_21
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745