| Literature DB >> 25577093 |
Patrícia Timóteo1, Anastasia Karioti2, Suzana G Leitão3, Franco Francesco Vincieri1, Anna Rita Bilia1.
Abstract
Infusions and decoctions of three chemotypes of Lippia alba (Mill.) N. E. Brown (Verbenaceae) were investigated for their quantitative profiles by HPLC-DAD-ESI-MS analyses. An RP-HPLC method was developed which permitted the quality control of the preparations. The correct choice of the column allowed the detailed characterization of the constituents in a total analysis time of 35 min. The HPLC method was accordingly validated for linearity range, LOD, LOQ, accuracy and precision. For the quantitative analysis the three major phytochemical groups were taken into consideration, namely iridoids, phenylpropanoids and flavonoids. Comparative quantitative analyses revealed significant differences among the chemotypes that should be taken into account in the uses of the herbal teas. The developed HPLC-UV assay proved to be an efficient and alternative method for the discrimination of the three chemotypes. This is the first report of detailed analysis of the chemical composition of the constituents of L. alba chemotypes' teas.Entities:
Keywords: Chemotypes; Flavonoid glycosides; HPLC–DAD–ESI-MS; Herbal teas; Iridoids; Lippia alba; Phenylpropanoid glycosides
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Year: 2014 PMID： 25577093 DOI： 10.1016/j.foodchem.2014.11.129
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514