Maksim Ionov1, Karol Ciepluch2, Zuzana Garaiova3, Sophie Melikishvili3, Sylwia Michlewska4, Łucja Balcerzak4, Sława Glińska4, Katarzyna Miłowska2, Rafael Gomez-Ramirez5, Francisco Javier de la Mata5, Dzmitry Shcharbin6, Iveta Waczulikova3, Maria Bryszewska2, Tibor Hianik3. 1. Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. Electronic address: maksion@biol.uni.lodz.pl. 2. Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. 3. Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina, 842 48 Bratislava, Slovakia. 4. Laboratory of Electron Microscopy, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Łódż, Poland. 5. Departamento Química Inorgánica, Universidad de Alcalá de Henares, CIBER-BBN Alcalá de Henares, Spain. 6. Institute of Biophysics and Cell Engineering of NASB, Minsk, Belarus.
Abstract
AIMS: We have investigated the effect of surface charge of model lipid membranes on their interactions with dendriplexes formed by HIV-derived peptides and 2 types of positively charged carbosilane dendrimers (CBD). METHODS: Interaction of dendriplexes with lipid membranes was measured by fluorescence anisotropy, dynamic light scattering and Langmuir-Blodgett techniques. The morphology of the complexes was examined by transmission electron microscopy. RESULTS: All dendriplexes independent of the type of peptide interacted with model lipid membranes. Negatively charged vesicles composed of a mixture of DMPC/DPPG interacted more strongly, and it was accompanied by an increase in anisotropy of the fluorescent probe localized in polar domain of lipid bilayers. There was also an increase in surface pressure of the lipid monolayers. Mixing negatively charged liposomes with dendriplexes increased liposome size and made their surface charges more positive. CONCLUSIONS: HIV-peptide/dendrimer complexes interact with model lipid membranes depending on their surface charge. Carbosilane dendrimers can be useful as non-viral carriers for delivering HIV-peptides into cells.
AIMS: We have investigated the effect of surface charge of model lipid membranes on their interactions with dendriplexes formed by HIV-derived peptides and 2 types of positively charged carbosilane dendrimers (CBD). METHODS: Interaction of dendriplexes with lipid membranes was measured by fluorescence anisotropy, dynamic light scattering and Langmuir-Blodgett techniques. The morphology of the complexes was examined by transmission electron microscopy. RESULTS: All dendriplexes independent of the type of peptide interacted with model lipid membranes. Negatively charged vesicles composed of a mixture of DMPC/DPPG interacted more strongly, and it was accompanied by an increase in anisotropy of the fluorescent probe localized in polar domain of lipid bilayers. There was also an increase in surface pressure of the lipid monolayers. Mixing negatively charged liposomes with dendriplexes increased liposome size and made their surface charges more positive. CONCLUSIONS: HIV-peptide/dendrimer complexes interact with model lipid membranes depending on their surface charge. Carbosilane dendrimers can be useful as non-viral carriers for delivering HIV-peptides into cells.
Authors: Marcin Hołota; Jakub Magiera; Sylwia Michlewska; Małgorzata Kubczak; Natalia Sanz Del Olmo; Sandra García-Gallego; Paula Ortega; F Javier de la Mata; Maksim Ionov; Maria Bryszewska Journal: Biomolecules Date: 2019-04-18
Authors: Sylwia Michlewska; Małgorzata Kubczak; Marta Maroto-Díaz; Natalia Sanz Del Olmo; Paula Ortega; Dzmitry Shcharbin; Rafael Gomez Ramirez; Francisco Javier de la Mata; Maksim Ionov; Maria Bryszewska Journal: Biomolecules Date: 2019-08-25