Robyn S Lee1, Nicolas Radomski2, Jean-Francois Proulx3, Jeremy Manry4, Fiona McIntosh2, Francine Desjardins5, Hafid Soualhine6, Pilar Domenech2, Michael B Reed7, Dick Menzies8, Marcel A Behr7. 1. Department of Epidemiology, Biostatistics and Occupational Health Department of McGill International TB Centre The Research Institute of the McGill University Health Centre. 2. The Research Institute of the McGill University Health Centre. 3. Nunavik Regional Board of Health and Social Services, Kuujjuaq. 4. Department of Medicine Department of Human Genetics, McGill University Department of McGill International TB Centre The Research Institute of the McGill University Health Centre. 5. Mycobacteriology Laboratory, Royal Victoria Hospital. 6. Laboratoire de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Québec, Canada. 7. Department of McGill International TB Centre The Research Institute of the McGill University Health Centre. 8. The Research Institute of the McGill University Health Centre Respiratory Epidemiology and Clinical Research Unit, Montreal Chest Institute, McGill University Health Centre.
Abstract
BACKGROUND: Between November 2011 and November 2012, a Canadian village of 933 persons had 50 culture-positive cases of tuberculosis, with 49 sharing the same genotype. METHODS: We performed Illumina-based whole-genome sequencing on Mycobacterium tuberculosis isolates from this village, during and before the outbreak. Phylogenetic trees were generated using the maximum likelihood method. RESULTS: Three distinct genotypes were identified. Strain I (n = 7) was isolated in 1991-1996. Strain II (n = 8) was isolated in 1996-2004. Strain III (n = 62) first appeared in 2007 and did not arise from strain I or II. Within strain III, there were 3 related but distinct clusters: IIIA, IIIB, and IIIC. Between 2007 and 2010, cluster IIIA predominated (11 of 22 vs 2 of 40; P < .001), whereas in 2011-2012 clusters IIIB (n = 18) and IIIC (n = 20) predominated over cluster IIIA (n = 11). Combined evolutionary and epidemiologic analysis of strain III cases revealed that the outbreak in 2011-2012 was the result of ≥6 temporally staggered events, spanning from 1 reactivation case to a point-source outbreak of 20 cases. CONCLUSIONS: After the disappearance of 2 strains of M. tuberculosis in this village, its reemergence in 2007 was followed by an epidemiologic amplification, affecting >5% of the population.
BACKGROUND: Between November 2011 and November 2012, a Canadian village of 933 persons had 50 culture-positive cases of tuberculosis, with 49 sharing the same genotype. METHODS: We performed Illumina-based whole-genome sequencing on Mycobacterium tuberculosis isolates from this village, during and before the outbreak. Phylogenetic trees were generated using the maximum likelihood method. RESULTS: Three distinct genotypes were identified. Strain I (n = 7) was isolated in 1991-1996. Strain II (n = 8) was isolated in 1996-2004. Strain III (n = 62) first appeared in 2007 and did not arise from strain I or II. Within strain III, there were 3 related but distinct clusters: IIIA, IIIB, and IIIC. Between 2007 and 2010, cluster IIIA predominated (11 of 22 vs 2 of 40; P < .001), whereas in 2011-2012 clusters IIIB (n = 18) and IIIC (n = 20) predominated over cluster IIIA (n = 11). Combined evolutionary and epidemiologic analysis of strain III cases revealed that the outbreak in 2011-2012 was the result of ≥6 temporally staggered events, spanning from 1 reactivation case to a point-source outbreak of 20 cases. CONCLUSIONS: After the disappearance of 2 strains of M. tuberculosis in this village, its reemergence in 2007 was followed by an epidemiologic amplification, affecting >5% of the population.
Authors: Faiz Ahmad Khan; Greg J Fox; Robyn S Lee; Mylene Riva; Andrea Benedetti; Jean-François Proulx; Shelley Jung; Karen Hornby; Marcel A Behr; Dick Menzies Journal: CMAJ Open Date: 2016-09-16
Authors: Robyn S Lee; Nicolas Radomski; Jean-Francois Proulx; Ines Levade; B Jesse Shapiro; Fiona McIntosh; Hafid Soualhine; Dick Menzies; Marcel A Behr Journal: Proc Natl Acad Sci U S A Date: 2015-10-19 Impact factor: 11.205
Authors: Gonzalo G Alvarez; Alice A Zwerling; Carla Duncan; Christopher Pease; Deborah Van Dyk; Marcel A Behr; Robyn S Lee; Sunita Mulpuru; Smita Pakhale; D William Cameron; Shawn D Aaron; Michael Patterson; Jean Allen; Kathryn Sullivan; Anne Jolly; Meenu K Sharma; Frances B Jamieson Journal: Clin Infect Dis Date: 2021-06-15 Impact factor: 9.079
Authors: Hollie-Ann Hatherell; Caroline Colijn; Helen R Stagg; Charlotte Jackson; Joanne R Winter; Ibrahim Abubakar Journal: BMC Med Date: 2016-03-23 Impact factor: 8.775