Literature DB >> 2557231

VIP receptors and control of short circuit current in the human intestinal clonal cell line Cl.19A.

C Rouyer-Fessard1, C Augeron, E Grasset, J J Maoret, C L Laboisse, M Laburthe.   

Abstract

At the maximally effective concentration of 10 nM, VIP induced a marked (12.5-fold stimulation above basal), and sustained increase in short circuit current in the human intestinal epithelial cell line Cl.19A grown on permeable filters and placed in Ussing chambers. Half-maximal increase of Isc was observed for 0.1 nM VIP. This was well correlated with the VIP-stimulated adenylate cyclase activity (ED50:0.07 nM). Binding studies using 125I-VIP indicated that Cl.19A cells express a peptide-specific VIP receptor with a dissociation constant of 0.07 nM. Covalent labeling of receptors followed by SDS-PAGE analysis of membrane proteins resulted in the identification of a 63,000 dalton binding protein in Cl.19A cells.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2557231     DOI： 10.1007/BF01950169

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  13 in total

1.  Vasoactive intestinal peptide: a potent stimulator of adenosine 3':5'-cyclic monophosphate accumulation in gut carcinoma cell lines in culture.

Authors:  M Laburthe; M Rousset; C Boissard; G Chevalier; A Zweibaum; G Rosselin
Journal:  Proc Natl Acad Sci U S A       Date:  1978-06       Impact factor: 11.205

2.  The human vasoactive intestinal peptide receptor: molecular identification by covalent cross-linking in colonic epithelium.

Authors:  A Couvineau; M Laburthe
Journal:  J Clin Endocrinol Metab       Date:  1985-07       Impact factor: 5.958

3.  Vasoactive intestinal polypeptide-induced chloride secretion by a colonic epithelial cell line. Direct participation of a basolaterally localized Na+,K+,Cl- cotransport system.

Authors:  K Dharmsathaphorn; K G Mandel; H Masui; J A McRoberts
Journal:  J Clin Invest       Date:  1985-02       Impact factor: 14.808

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.

Authors:  T R Chen
Journal:  Exp Cell Res       Date:  1977-02       Impact factor: 3.905

6.  Epithelial properties of human colonic carcinoma cell line Caco-2: effect of secretagogues.

Authors:  E Grasset; J Bernabeu; M Pinto
Journal:  Am J Physiol       Date:  1985-05

7.  Study of species specificity in growth hormone-releasing factor (GRF) interaction with vasoactive intestinal peptide (VIP) receptors using GRF and intestinal VIP receptors from rat and human: evidence that Ac-Tyr1hGRF is a competitive VIP antagonist in the rat.

Authors:  M Laburthe; A Couvineau; C Rouyer-Fessard
Journal:  Mol Pharmacol       Date:  1986-01       Impact factor: 4.436

8.  Functional VIP receptors in the human mucus-secreting colonic epithelial cell line CL.16E.

Authors:  M Laburthe; C Augeron; C Rouyer-Fessard; I Roumagnac; J J Maoret; E Grasset; C Laboisse
Journal:  Am J Physiol       Date:  1989-03

9.  Vasoactive intestinal peptide (VIP): variation of the jejuno-ileal content in the developing rat as measured by radioreceptorassay.

Authors:  M Laburthe; D Bataille; G Rosselin
Journal:  Acta Endocrinol (Copenh)       Date:  1977-03

10.  Epithelial properties of human colonic carcinoma cell line Caco-2: electrical parameters.

Authors:  E Grasset; M Pinto; E Dussaulx; A Zweibaum; J F Desjeux
Journal:  Am J Physiol       Date:  1984-09
View more
  7 in total

Review 1.  Pathogenesis of human enterovirulent bacteria: lessons from cultured, fully differentiated human colon cancer cell lines.

Authors:  Vanessa Liévin-Le Moal; Alain L Servin
Journal:  Microbiol Mol Biol Rev       Date:  2013-09       Impact factor: 11.056

2.  MicroRNAs determine human intestinal epithelial cell fate.

Authors:  Guillaume Dalmasso; Hang Thi Thu Nguyen; Yutao Yan; Hamed Laroui; Shanthi Srinivasan; Shanthi V Sitaraman; Didier Merlin
Journal:  Differentiation       Date:  2010-07-17       Impact factor: 3.880

3.  Relation between chloride secretion and intracellular cyclic adenosine monophosphate in a cloned human intestinal cell line HT-29 cl 19A.

Authors:  S K Nath; X Huang; A L'helgoualc'h; M Rautureau; A Bisalli; M Heyman; J F Desjeux
Journal:  Gut       Date:  1994-05       Impact factor: 23.059

4.  Gastrointestinal dysfunction in mice with a targeted mutation in the gene encoding vasoactive intestinal polypeptide: a model for the study of intestinal ileus and Hirschsprung's disease.

Authors:  V Lelievre; G Favrais; C Abad; H Adle-Biassette; Y Lu; P M Germano; G Cheung-Lau; J R Pisegna; P Gressens; G Lawson; J A Waschek
Journal:  Peptides       Date:  2007-05-18       Impact factor: 3.750

5.  ATP-stimulated electrolyte and mucin secretion in the human intestinal goblet cell line HT29-Cl.16E.

Authors:  D Merlin; C Augeron; X Y Tien; X Guo; C L Laboisse; U Hopfer
Journal:  J Membr Biol       Date:  1994-01       Impact factor: 1.843

Review 6.  The Neuromodulation of the Intestinal Immune System and Its Relevance in Inflammatory Bowel Disease.

Authors:  Martina Di Giovangiulio; Simon Verheijden; Goele Bosmans; Nathalie Stakenborg; Guy E Boeckxstaens; Gianluca Matteoli
Journal:  Front Immunol       Date:  2015-11-20       Impact factor: 7.561

7.  The double stranded RNA analog poly-IC elicits both robust IFN-λ production and oncolytic activity in human gastrointestinal cancer cells.

Authors:  Chantal Bou-Hanna; Anne Jarry; Jean-François Mosnier; Céline Bossard; Christian L Laboisse
Journal:  Oncotarget       Date:  2018-10-02
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.