Xiao-Jin Wu1, Xiao-Dong Li2, Hanze Zhang3, Xiaofei Zhang4, Zhong-Hua Ning5, Yong-Mei Yin6, Ye Tian7. 1. Department of Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, China Department of Radiation Oncology, The First People's Hospital of Xuzhou, Xuzhou, China. 2. Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, China. 3. Department of Epidemiology and Biostatistics, College of Public Health, University of South Florida, Tampa, FL, USA. 4. Department of Clinical Oncology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan. 5. Department of Radiation Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, China. 6. Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. 7. Department of Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, China dryetian@hotmail.com.
Abstract
OBJECTIVES: To evaluate levels of CD44 standard variant (CD44s), CD44 variant exon 3 (CD44v3) and CD44 variant exon 6 (CD44v6) protein in breast cancer tissue, and investigate their relationships with clinicopathological characteristics of the disease. METHODS: Immunohistochemistry for CD44s, CD44v3 and CD44v6 was retrospectively performed on formalin-fixed paraffin wax-embedded breast cancer tissue samples. RESULTS: Tumour tissue samples from 60 patients with breast cancer were included. There was a significant relationship between CD44s positivity and tumour diameter and lymph node involvement. CD44v6 positivity was significantly associated with tumour-node-metastasis (TNM) stage and lymph node involvement. There were significant negative correlations between CD44s immunopositivity, tumour diameter and TNM stage, and significant positive correlations between CD44v6 immunopositivity, tumour diameter and TNM stage. CONCLUSIONS: CD44s and CD44v6 appear to play opposing roles in the development of breast cancer, but their precise functions and mechanisms of action remain unclear.
OBJECTIVES: To evaluate levels of CD44 standard variant (CD44s), CD44 variant exon 3 (CD44v3) and CD44 variant exon 6 (CD44v6) protein in breast cancer tissue, and investigate their relationships with clinicopathological characteristics of the disease. METHODS: Immunohistochemistry for CD44s, CD44v3 and CD44v6 was retrospectively performed on formalin-fixed paraffin wax-embedded breast cancer tissue samples. RESULTS:Tumour tissue samples from 60 patients with breast cancer were included. There was a significant relationship between CD44s positivity and tumour diameter and lymph node involvement. CD44v6 positivity was significantly associated with tumour-node-metastasis (TNM) stage and lymph node involvement. There were significant negative correlations between CD44s immunopositivity, tumour diameter and TNM stage, and significant positive correlations between CD44v6 immunopositivity, tumour diameter and TNM stage. CONCLUSIONS: CD44s and CD44v6 appear to play opposing roles in the development of breast cancer, but their precise functions and mechanisms of action remain unclear.
Authors: Tony Navas; Thomas D Pfister; Simona Colantonio; Amina Aziz; Lynda Dieckman; Richard G Saul; Jan Kaczmarczyk; Suzanne Borgel; Sergio Y Alcoser; Melinda G Hollingshead; Young H Lee; Donald P Bottaro; Tara Hiltke; Gordon Whiteley; Naoko Takebe; Robert J Kinders; Ralph E Parchment; Joseph E Tomaszewski; James H Doroshow Journal: PLoS One Date: 2018-06-21 Impact factor: 3.240