Literature DB >> 2557022

Separate regulatory control of apical and basolateral Na+/H+ exchange in renal epithelial cells.

V Casavola1, C Helmle-Kolb, H Murer.   

Abstract

Epithelial layers of LLC-PK1/PKE20 cells, a renal epithelial cell line which expresses Na+/H+ exchange activities in the apical as well as basolateral membrane domains, are examined in the single cell mode by microspectrofluorometry. We provide evidence that basolateral Na+/H+ exchange is more sensitive to amiloride inhibition than is apical Na+/H+ exchange. Furthermore, we demonstrate that the two exchange activities differ in their regulatory control: kinase A activation (forskolin, 8-Br-cAMP) leads to inhibition of both exchange activities, whereas kinase C activation (phorbol ester) stimulates basolateral and inhibits apical Na+/H+ exchange. Thus, renal epithelial cells may contain two Na+/H+ exchange activities: an apical ("epithelial") and basolateral ("housekeeping") which may serve different cellular functions and are under separate regulatory controls.

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Year:  1989        PMID： 2557022     DOI： 10.1016/s0006-291x(89)80041-5

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  24 in total

1.  Aldosterone actions on basolateral Na+/H+ exchange in Madin-Darby canine kidney cells.

Authors:  S Vilella; L Guerra; C Helmle-Kolb; H Murer
Journal:  Pflugers Arch       Date:  1992-10       Impact factor: 3.657

2.  Characterization of basolateral Na/H exchange (Na/H-1) in MDCK cells.

Authors:  S Vilella; L Guerra; C Helmle-Kolb; H Murer
Journal:  Pflugers Arch       Date:  1992-03       Impact factor: 3.657

3.  Studies on the kinetics of Na+/H+ exchange in OK cells: introduction of a new device for the analysis of polarized transport in cultured epithelia.

Authors:  D Krayer-Pawlowska; C Helmle-Kolb; M H Montrose; R Krapf; H Murer
Journal:  J Membr Biol       Date:  1991-03       Impact factor: 1.843

4.  Polarized expression of Na+/H+ exchange activities in clonal LLC-PK1 cells (Clone4 and PKE20) I. Basic characterization.

Authors:  V Casavola; C Helmle-Kolb; M H Montrose; H Murer
Journal:  Pflugers Arch       Date:  1991-04       Impact factor: 3.657

Review 5.  The role of ion antiporters in the maintenance of intracellular pH in rat vascular smooth muscle cells.

Authors:  D Hogue; M Michalak; L Fliegel
Journal:  Mol Cell Biochem       Date:  1991-04-10       Impact factor: 3.396

6.  Activation of protein kinase A acutely inhibits and phosphorylates Na/H exchanger NHE-3.

Authors:  O W Moe; M Amemiya; Y Yamaji
Journal:  J Clin Invest       Date:  1995-11       Impact factor: 14.808

7.  Relative sensitivity to inhibition by cimetidine and clonidine differentiates between the two types of Na(+)-H+ exchangers in cultured cells.

Authors:  S Ramamoorthy; C Tiruppathi; C N Nair; V B Mahesh; F H Leibach; V Ganapathy
Journal:  Biochem J       Date:  1991-12-01       Impact factor: 3.857

8.  Functional adaptation to high PCO2 of apically and basolaterally located Na+/H+ exchange activities in cultured renal cell lines.

Authors:  B Mrkic; C Helmle-Kolb; R Krapf; H Murer
Journal:  Pflugers Arch       Date:  1994-02       Impact factor: 3.657

9.  Na+/H(+)-exchange in A6 cells: polarity and vasopressin regulation.

Authors:  V Casavola; L Guerra; C Helmle-Kolb; S J Reshkin; H Murer
Journal:  J Membr Biol       Date:  1992-11       Impact factor: 1.843

10.  Carbachol- and elevated Ca(2+)-induced translocation of functionally active protein kinase C to the brush border of rabbit ileal Na+ absorbing cells.

Authors:  M E Cohen; J Wesolek; J McCullen; K Rys-Sikora; S Pandol; R P Rood; G W Sharp; M Donowitz
Journal:  J Clin Invest       Date:  1991-09       Impact factor: 14.808
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