Wei-Sheng Chen1, Zhiyi Cao2, Laetitia Truong3, Satoshi Sugaya2, Noorjahan Panjwani4. 1. Program in Cell, Molecular & Developmental Biology, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, Massachusetts, United States. 2. New England Eye Center/Department of Ophthalmology, Tufts University, Boston, Massachusetts, United States. 3. Public Health and Professional Degree Programs, Tufts University School of Medicine, Boston, Massachusetts, United States. 4. Program in Cell, Molecular & Developmental Biology, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, Massachusetts, United States New England Eye Center/Department of Ophthalmology, Tufts University, Boston, Massachusetts, United States Department of Developmental, Molecular and Chemical Biology, Tufts University, Boston, Massachusetts, United States.
Abstract
PURPOSE: In this study, we aimed to assess whether the expression pattern of galectins is altered in Pseudomonas aeruginosa-infected and chemically burned mouse corneas. METHODS: Galectin (Gal) fingerprinting of normal, P. aeruginosa-infected, and silver nitrate-cauterized corneas was performed by Western blotting, immunofluorescence staining, and qRT-PCR. RESULTS: In normal corneas, Gal-1 was distributed mainly in the stroma, Gal-3 was localized mainly in epithelium, and Gal-7, -8, and -9 were detected in both corneal epithelium and stroma. Expression levels of the five galectins were drastically altered under pathological conditions. In both infected and cauterized corneas, overall Gal-3 expression was downregulated, whereas overall Gal-8 and -9 were upregulated. Changes in the expression level of Gal-7, -8, and -9 were distinct in the epithelium of infected and cauterized corneas. Expression of these three galectins was upregulated in corneal epithelium of infected corneas but not in cauterized corneas. Consistent with the changes in protein expression: (1) Gal-7, -8, and -9 mRNA expression was upregulated in cauterized corneas, and (2) Gal-3 mRNA was downregulated and Gal-9 mRNA expression was upregulated in infected corneas. CONCLUSIONS: Our data demonstrate differential regulation of various members of the galectin family in the course of corneal infection and neovascularization. The emerging functionality of the sugar code of cell surface receptors via endogenous galectins reflect to the pertinent roles of the five tested galectins in the diseases of cornea. Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.
PURPOSE: In this study, we aimed to assess whether the expression pattern of galectins is altered in Pseudomonas aeruginosa-infected and chemically burned mouse corneas. METHODS: Galectin (Gal) fingerprinting of normal, P. aeruginosa-infected, and silver nitrate-cauterized corneas was performed by Western blotting, immunofluorescence staining, and qRT-PCR. RESULTS: In normal corneas, Gal-1 was distributed mainly in the stroma, Gal-3 was localized mainly in epithelium, and Gal-7, -8, and -9 were detected in both corneal epithelium and stroma. Expression levels of the five galectins were drastically altered under pathological conditions. In both infected and cauterized corneas, overall Gal-3 expression was downregulated, whereas overall Gal-8 and -9 were upregulated. Changes in the expression level of Gal-7, -8, and -9 were distinct in the epithelium of infected and cauterized corneas. Expression of these three galectins was upregulated in corneal epithelium of infected corneas but not in cauterized corneas. Consistent with the changes in protein expression: (1) Gal-7, -8, and -9 mRNA expression was upregulated in cauterized corneas, and (2) Gal-3 mRNA was downregulated and Gal-9 mRNA expression was upregulated in infected corneas. CONCLUSIONS: Our data demonstrate differential regulation of various members of the galectin family in the course of corneal infection and neovascularization. The emerging functionality of the sugar code of cell surface receptors via endogenous galectins reflect to the pertinent roles of the five tested galectins in the diseases of cornea. Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.
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