| Literature DB >> 2556424 |
J K Li1, T Johnson, Y Y Yang, V Shore.
Abstract
The total viral structural polypeptides and the double-stranded genomic RNAs of bluetongue virus can be selectively separated by a single SDS-KCl precipitation step. This simple, rapid and highly reproducible method enables greater than 95% recovery and purity of both viral proteins and dsRNAs within 30 min. The serotypic identity of the separated dsRNAs can be analyzed by SDS-PAGE electrophorogram immediately. After a single phenol/chloroform extraction, the dsRNA can also be used as hybridization probes, templates for molecular cloning and direct RNA sequencing. The SDS-KCl-precipitated viral proteins could be used readily for peptide mapping and as immunogens. Polyclonal and monoclonal antibodies raised against SDS-KCl-precipitated viral structural polypeptides were useful in Western immunoblots.Entities:
Mesh：
Substances：
Year: 1989 PMID： 2556424 DOI： 10.1016/0166-0934(89)90069-4
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014