Chunyang Dai1, Yan Zhang2, Xiaoling Ma3, Meiling Yin2, Haiyang Zheng4, Xuejun Gu4, Shaoqing Xie4, Hengmin Jia5, Liang Zhang5, Weijun Zhang4. 1. Department of Laboratory Medicine, Anhui Provincial Hospital, Anhui Medical University, Hefei, China. 2. Department of Laboratory Medicine, Anhui Provincial Hospital, Hefei, China. 3. Department of Laboratory Medicine, Anhui Provincial Hospital, Anhui Medical University, Hefei, China. Electronic address: xiaolingma@126.com. 4. Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, China. 5. Department of Nosocomial Infection, Anhui Provincial Hospital, Hefei, China.
Abstract
BACKGROUND: Airborne bacterial contamination poses a risk for surgical site infection, and routine surveillance of airborne bacteria is important. Traditional methods for detecting airborne bacteria are time consuming and strenuous. Measurement of biologic particle concentrations using a fluorescent particle counter is a novel method for evaluating air quality. The current study was to determine whether the number of biologic particles detected by the fluorescent particle counter can be used to indicate airborne bacterial counts in operating rooms. METHODS: The study was performed in an operating theater at a university hospital in Hefei, China. The number of airborne biologic particles every minute was quantified using a fluorescent particle counter. Microbiologic air sampling was performed every 30 minutes using an Andersen air sampler (Pusong Electronic Instruments, Changzhou, China). Correlations between the 2 different methods were analyzed by Pearson correlation coefficients. RESULTS: A significant correlation was observed between biologic particle and bacterial counts (Pearson correlation coefficient = 0.76), and the counting results from 2 methods both increased substantially between operations, corresponding with human movements in the operating room. CONCLUSION: Fluorescent particle counters show potential as important tools for monitoring bacterial contamination in operating theatres.
BACKGROUND: Airborne bacterial contamination poses a risk for surgical site infection, and routine surveillance of airborne bacteria is important. Traditional methods for detecting airborne bacteria are time consuming and strenuous. Measurement of biologic particle concentrations using a fluorescent particle counter is a novel method for evaluating air quality. The current study was to determine whether the number of biologic particles detected by the fluorescent particle counter can be used to indicate airborne bacterial counts in operating rooms. METHODS: The study was performed in an operating theater at a university hospital in Hefei, China. The number of airborne biologic particles every minute was quantified using a fluorescent particle counter. Microbiologic air sampling was performed every 30 minutes using an Andersen air sampler (Pusong Electronic Instruments, Changzhou, China). Correlations between the 2 different methods were analyzed by Pearson correlation coefficients. RESULTS: A significant correlation was observed between biologic particle and bacterial counts (Pearson correlation coefficient = 0.76), and the counting results from 2 methods both increased substantially between operations, corresponding with human movements in the operating room. CONCLUSION: Fluorescent particle counters show potential as important tools for monitoring bacterial contamination in operating theatres.
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