Literature DB >> 25563178

Ca2+ mobilization assays in GPCR drug discovery.

Grzegorz Woszczek1, Elisabeth Fuerst.   

Abstract

Intracellular calcium mobilization can be measured using several methods varying in indicator dyes and devices used. In this chapter, we describe the fluorescence-based method (FLIPR Calcium 4 Assay) developed by Molecular Devices for a FlexStation and routinely used in our laboratory for detecting intracellular calcium changes. The assay is designed to study calcium mobilization induced by majority of GPCRs and calcium channels and allows for simultaneous concentration-dependent analysis of several receptor agonists and antagonists, useful in receptor characterization and drug discovery projects.

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Year:  2015        PMID： 25563178     DOI： 10.1007/978-1-4939-2336-6_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Thieno[2,3-d]pyrimidine-Based Positive Allosteric Modulators of Human Mas-Related G Protein-Coupled Receptor X1 (MRGPRX1).

Authors:  Ilyas Berhane; Niyada Hin; Ajit G Thomas; Qian Huang; Chi Zhang; Vijayabhaskar Veeravalli; Ying Wu; Justin Ng; Jesse Alt; Camilo Rojas; Hiroe Hihara; Mika Aoki; Kyoko Yoshizawa; Tomoki Nishioka; Shuichi Suzuki; Shao-Qiu He; Qi Peng; Yun Guan; Xinzhong Dong; Srinivasa N Raja; Barbara S Slusher; Rana Rais; Takashi Tsukamoto
Journal:  J Med Chem       Date:  2022-02-04       Impact factor: 8.039

2.  Development of a High-Throughput Calcium Mobilization Assay for CCR6 Receptor Coupled to Hydrolase Activity Readout.

Authors:  Sara Gómez-Melero; Fé Isabel García-Maceira; Tania García-Maceira; Verónica Luna-Guerrero; Gracia Montero-Peñalvo; Javier Caballero-Villarraso; Isaac Túnez; Elier Paz-Rojas
Journal:  Biomedicines       Date:  2022-02-10

3.  Leukotriene E4 is a full functional agonist for human cysteinyl leukotriene type 1 receptor-dependent gene expression.

Authors:  Holly R Foster; Elisabeth Fuerst; William Branchett; Tak H Lee; David J Cousins; Grzegorz Woszczek
Journal:  Sci Rep       Date:  2016-02-02       Impact factor: 4.379
  3 in total

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