Anas A Saeed1, Andrew H Sims2, Stephen S Prime3, Ian Paterson4, Paul G Murray5, Victor R Lopes6. 1. Department of Oral Surgery, Edinburgh Postgraduate Dental Institute, University of Edinburgh, Lauriston Place, Lauriston Building, EH3 9HA Edinburgh, UK. Electronic address: anas.saeed@ed.ac.uk. 2. Applied Bioinformatics of Cancer, University of Edinburgh Cancer Research Centre, Crewe Road South, Edinburgh EH4 2XR, UK. Electronic address: andrew.sims@ed.ac.uk. 3. Centre for Clinical and Diagnostic Oral Sciences, Institute of Dentistry, Barts and the London School of Dentistry, Queen Mary University of London, Turner Street, Whitechapel E1 2AD, UK. Electronic address: stephensprime@gmail.com. 4. Dental Research and Training Unit, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: ipaterson@um.edu.my. 5. School of Cancer Sciences, Birmingham Cancer Research UK Centre, Birmingham University, Edgbaston B15 2TT, Birmingham, UK. Electronic address: p.g.murray@bham.ac.uk. 6. Head and Neck Directorate NHS Lothian and University of Edinburgh, UK. Electronic address: vlopes@staffmail.ed.ac.uk.
Abstract
OBJECTIVES: It is well recognized that oral squamous cell carcinoma (OSCC) cases from Asia that are associated with betel quid chewing are phenotypically distinct to those from Western countries that are predominantly caused by smoking/drinking, but the molecular basis of these differences are largely unknown. The aim of this study is to examine gene expression, related carcinogenic pathways and molecular processes that might be responsible for the phenotypic heterogeneity of OSCC between UK and Sri Lankan population groups. METHODS: We have compared the gene expression profiles of OSCCs and normal oral mucosal tissues from both Sri Lankan and UK individuals using Affymetrix gene expression arrays. The generated data was interrogated using significance analysis of microarrays and Ingenuity Pathway Analysis (IPA). RESULTS: The gene expression profiles of UK and Sri Lankan OSCC are similar in many respects to other oral cancer expression profiles reported in the literature and were mainly similar to each other. However, genes involved in tumor invasion, metastasis and recurrence were more obviously associated with UK tumors as opposed to those from Sri Lanka. CONCLUSION: The development of OSCCs in both UK and Sri Lankan populations appears largely mediated by similar biological pathways despite the differences related to race, ethnicity, lifestyle, and/or exposure to environmental carcinogens. However, IPA revealed a highly activated "Cell-mediated Immune Response" in Sri Lankan normal and tumor samples relative to UK cohorts. It seems likely, therefore, that any future attempts to personalize treatment for OSCC patients will need to be different in Western and Asian countries to reflect differences in gene expression and the immune status of the patients.
OBJECTIVES: It is well recognized that oral squamous cell carcinoma (OSCC) cases from Asia that are associated with betel quid chewing are phenotypically distinct to those from Western countries that are predominantly caused by smoking/drinking, but the molecular basis of these differences are largely unknown. The aim of this study is to examine gene expression, related carcinogenic pathways and molecular processes that might be responsible for the phenotypic heterogeneity of OSCC between UK and Sri Lankan population groups. METHODS: We have compared the gene expression profiles of OSCCs and normal oral mucosal tissues from both Sri Lankan and UK individuals using Affymetrix gene expression arrays. The generated data was interrogated using significance analysis of microarrays and Ingenuity Pathway Analysis (IPA). RESULTS: The gene expression profiles of UK and Sri Lankan OSCC are similar in many respects to other oral cancer expression profiles reported in the literature and were mainly similar to each other. However, genes involved in tumor invasion, metastasis and recurrence were more obviously associated with UK tumors as opposed to those from Sri Lanka. CONCLUSION: The development of OSCCs in both UK and Sri Lankan populations appears largely mediated by similar biological pathways despite the differences related to race, ethnicity, lifestyle, and/or exposure to environmental carcinogens. However, IPA revealed a highly activated "Cell-mediated Immune Response" in Sri Lankan normal and tumor samples relative to UK cohorts. It seems likely, therefore, that any future attempts to personalize treatment for OSCC patients will need to be different in Western and Asian countries to reflect differences in gene expression and the immune status of the patients.
Authors: Sathya Narayanan Patmanathan; Steven P Johnson; Sook Ling Lai; Suthashini Panja Bernam; Victor Lopes; Wenbin Wei; Maha Hafez Ibrahim; Federico Torta; Pradeep Narayanaswamy; Markus R Wenk; Deron R Herr; Paul G Murray; Lee Fah Yap; Ian C Paterson Journal: Sci Rep Date: 2016-05-10 Impact factor: 4.379
Authors: Jean-Philippe Foy; Chloé Bertolus; David Boutolleau; Henri Agut; Antoine Gessain; Zdenko Herceg; Pierre Saintigny Journal: Front Oncol Date: 2020-05-21 Impact factor: 6.244