| Literature DB >> 25560303 |
Jung-Chien Cheng1, Hsun-Ming Chang, Lanlan Fang, Ying-Pu Sun, Peter C K Leung.
Abstract
Connexin43 (Cx43)-mediated gap junctional intercellular communication (GJIC) are required for human trophoblast differentiation. To date, whether Cx43 mediates TGF-β1-induced trophoblast differentiation has not been determined. We showed that treatment with TGF-β1 increased Cx43 expression and GJIC in HTR-8/SVneo human trophoblast cells. In addition, Smad and ERK1/2 signaling pathways were involved in TGF-β1-induced up-regulation of Cx43. Moreover, TGF-β1 increased the expression of the syncytiotrophoblast marker, β-hCG. Importantly, knockdown of Cx43 abolished the TGF-β1-induced up-regulation of β-hCG. Furthermore, overexpression of Cx43 up-regulated β-hCG expression. These results provide evidence that Cx43 and GJIC activity are up-regulated by TGF-β1 in human trophoblast cells, which subsequently contributes to TGF-β1-induced trophoblast differentiation.Entities:
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Year: 2015 PMID： 25560303 DOI： 10.1002/jcp.24902
Source DB: PubMed Journal: J Cell Physiol ISSN： 0021-9541 Impact factor: 6.384