Literature DB >> 25560064

How FlAsH got its sparkle: historical recollections of the biarsenical-tetracysteine tag.

B Albert Griffin1, Stephen R Adams, Roger Y Tsien.   

Abstract

The biarsenical-tetracysteine tagging system was the first of (and inspiration for) the now numerous methods for site-specifically labeling proteins in living cells with small molecules such as fluorophores. This historical recollection describes its conception and the trial-and-error chemical development required to become a versatile technique.

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Year:  2015        PMID: 25560064     DOI: 10.1007/978-1-4939-2272-7_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  2 in total

1.  Rotamer-Restricted Fluorogenicity of the Bis-Arsenical ReAsH.

Authors:  Allison S Walker; Paul R Rablen; Alanna Schepartz
Journal:  J Am Chem Soc       Date:  2016-05-10       Impact factor: 15.419

2.  Distance-Matched Tagging Sequence Optimizes Live-Cell Protein Labeling by a Biarsenical Fluorescent Reagent AsCy3_E.

Authors:  Karen A Hecht; Yijia Xiong; Daniel A Barrack; Nicole R Ford; Guritno Roesijadi; Thomas C Squier
Journal:  ACS Omega       Date:  2018-02-21
  2 in total

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