Literature DB >> 25559107

RNA Interference in Caenorhabditis elegans.

Darryl Conte1, Lesley T MacNeil2, Albertha J M Walhout2, Craig C Mello1,3.   

Abstract

RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA, and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens.
Copyright © 2015 John Wiley & Sons, Inc.

Entities:  

Keywords:  C. elegans; RNAi; feeding; high-throughput screening assays; microinjection; soaking

Mesh：

Year:  2015        PMID： 25559107      PMCID： PMC5396541          DOI： 10.1002/0471142727.mb2603s109

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  44 in total

1.  Functional anatomy of a dsRNA trigger: differential requirement for the two trigger strands in RNA interference.

Authors:  S Parrish; J Fleenor; S Xu; C Mello; A Fire
Journal:  Mol Cell       Date:  2000-11       Impact factor: 17.970

2.  RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions.

Authors:  Ben Lehner; Julia Tischler; Andrew G Fraser
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

3.  RNAi in C. elegans: soaking in the genome sequence.

Authors:  H Tabara; A Grishok; C C Mello
Journal:  Science       Date:  1998-10-16       Impact factor: 47.728

4.  Subcellular in vivo time-lapse imaging and optical manipulation of Caenorhabditis elegans in standard multiwell plates.

Authors:  Christopher B Rohde; Mehmet Fatih Yanik
Journal:  Nat Commun       Date:  2011       Impact factor: 14.919

5.  Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.

Authors:  A Fire; S Xu; M K Montgomery; S A Kostas; S E Driver; C C Mello
Journal:  Nature       Date:  1998-02-19       Impact factor: 49.962

6.  Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans.

Authors:  B Sönnichsen; L B Koski; A Walsh; P Marschall; B Neumann; M Brehm; A-M Alleaume; J Artelt; P Bettencourt; E Cassin; M Hewitson; C Holz; M Khan; S Lazik; C Martin; B Nitzsche; M Ruer; J Stamford; M Winzi; R Heinkel; M Röder; J Finell; H Häntsch; S J M Jones; M Jones; F Piano; K C Gunsalus; K Oegema; P Gönczy; A Coulson; A A Hyman; C J Echeverri
Journal:  Nature       Date:  2005-03-24       Impact factor: 49.962

7.  RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans.

Authors:  F Piano; A J Schetter; M Mangone; L Stein; K J Kemphues
Journal:  Curr Biol       Date:  2000 Dec 14-28       Impact factor: 10.834

8.  SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C. elegans embryos.

Authors:  Yanxia Bei; Jennifer Hogan; Laura A Berkowitz; Martha Soto; Christian E Rocheleau; Ka Ming Pang; John Collins; Craig C Mello
Journal:  Dev Cell       Date:  2002-07       Impact factor: 12.270

9.  Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes.

Authors:  Kaveh Ashrafi; Francesca Y Chang; Jennifer L Watts; Andrew G Fraser; Ravi S Kamath; Julie Ahringer; Gary Ruvkun
Journal:  Nature       Date:  2003-01-16       Impact factor: 49.962

10.  Novel insights into RNAi off-target effects using C. elegans paralogs.

Authors:  Jean-François Rual; Niels Klitgord; Guillaume Achaz
Journal:  BMC Genomics       Date:  2007-04-19       Impact factor: 3.969
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  24 in total

1.  NHJ-1 Is Required for Canonical Nonhomologous End Joining in Caenorhabditis elegans.

Authors:  Aleksandar Vujin; Steven J Jones; Monique Zetka
Journal:  Genetics       Date:  2020-05-26       Impact factor: 4.562

2.  A developmental gene regulatory network for C. elegans anchor cell invasion.

Authors:  Taylor N Medwig-Kinney; Jayson J Smith; Nicholas J Palmisano; Sujata Tank; Wan Zhang; David Q Matus
Journal:  Development       Date:  2020-01-02       Impact factor: 6.868

3.  Physical exertion exacerbates decline in the musculature of an animal model of Duchenne muscular dystrophy.

Authors:  K J Hughes; A Rodriguez; K M Flatt; S Ray; A Schuler; B Rodemoyer; V Veerappan; K Cuciarone; A Kullman; C Lim; N Gutta; S Vemuri; V Andriulis; D Niswonger; L Barickman; W Stein; A Singhvi; N E Schroeder; A G Vidal-Gadea
Journal:  Proc Natl Acad Sci U S A       Date:  2019-02-12       Impact factor: 11.205

4.  A metabolic regulatory network for the Caenorhabditis elegans intestine.

Authors:  Sushila Bhattacharya; Brent B Horowitz; Jingyan Zhang; Xuhang Li; Hefei Zhang; Gabrielle E Giese; Amy D Holdorf; Albertha J M Walhout
Journal:  iScience       Date:  2022-06-30

5.  Inhibition of histone H3-H4 chaperone pathways rescues C. elegans sterility by H2B loss.

Authors:  Ruixue Zhao; Zhiwen Zhu; Ruxu Geng; Xuguang Jiang; Wei Li; Guangshuo Ou
Journal:  PLoS Genet       Date:  2022-06-09       Impact factor: 6.020

6.  Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans.

Authors:  Meghan Lee Arnold; Jason Cooper; Barth D Grant; Monica Driscoll
Journal:  J Vis Exp       Date:  2020-09-18       Impact factor: 1.355

7.  The RNase PARN-1 Trims piRNA 3' Ends to Promote Transcriptome Surveillance in C. elegans.

Authors:  Wen Tang; Shikui Tu; Heng-Chi Lee; Zhiping Weng; Craig C Mello
Journal:  Cell       Date:  2016-02-25       Impact factor: 41.582

Review 8.  Loss-of-function genetic tools for animal models: cross-species and cross-platform differences.

Authors:  Benjamin E Housden; Matthias Muhar; Matthew Gemberling; Charles A Gersbach; Didier Y R Stainier; Geraldine Seydoux; Stephanie E Mohr; Johannes Zuber; Norbert Perrimon
Journal:  Nat Rev Genet       Date:  2016-10-31       Impact factor: 53.242

9.  Chemical and genetic rescue of in vivo progranulin-deficient lysosomal and autophagic defects.

Authors:  James J Doyle; Claudia Maios; Céline Vrancx; Sarah Duhaime; Babykumari Chitramuthu; Hugh P J Bennett; Andrew Bateman; J Alex Parker
Journal:  Proc Natl Acad Sci U S A       Date:  2021-06-22       Impact factor: 11.205

10.  Natural genetic variation drives microbiome selection in the Caenorhabditis elegans gut.

Authors:  Fan Zhang; Jessica L Weckhorst; Adrien Assié; Ciara Hosea; Christopher A Ayoub; Anastasia S Khodakova; Mario Loeza Cabrera; Daniela Vidal Vilchis; Marie-Anne Félix; Buck S Samuel
Journal:  Curr Biol       Date:  2021-05-27       Impact factor: 10.834
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