Literature DB >> 2555292

An improved nuclear extract preparation method.

K Zerivitz1, G Akusjärvi.   

Abstract

A rapid, efficient, and highly reproducible procedure for nuclear extract preparation is described. The method uses lysolecithin (lysophosphatidylcholine) to disrupt plasma membranes and requires no detergents or douncing. Soluble extracts prepared by this method are comparable to conventional nuclear extracts in all assays tested. Lysolecithin nuclear extracts are competent for RNA polymerase II and III transcription, DNA replication, pre-mRNA splicing, and sequence specific DNA-protein binding. Nuclear extracts can be prepared on a small scale (10(7) cells) as well as for preparative purposes by this method.

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Year:  1989        PMID： 2555292     DOI： 10.1016/0735-0651(89)90016-2

Source DB:  PubMed          Journal:  Gene Anal Tech        ISSN： 0735-0651


  11 in total

1.  Annotated proteome of a human T-cell lymphoma.

Authors:  David K Crockett; Charles E Seiler; Kojo S J Elenitoba-Johnson; Megan S Lim
Journal:  J Biomol Tech       Date:  2005-12

2.  Vitamin D and gonadal steroid-resistant New World primate cells express an intracellular protein which competes with the estrogen receptor for binding to the estrogen response element.

Authors:  H Chen; J E Arbelle; M A Gacad; E A Allegretto; J S Adams
Journal:  J Clin Invest       Date:  1997-02-15       Impact factor: 14.808

3.  Perlecan regulates Oct-1 gene expression in vascular smooth muscle cells.

Authors:  M C Weiser; N A Grieshaber; P E Schwartz; R A Majack
Journal:  Mol Biol Cell       Date:  1997-06       Impact factor: 4.138

4.  Characterization of factors involved in regulated adenovirus-2 major late pre-mRNA splicing.

Authors:  K Nordqvist; K Zerivitz; J P Kreivi; K Ohman; G Akusjärvi
Journal:  Mol Biol Rep       Date:  1990       Impact factor: 2.316

5.  Inhibition of nuclear import by protein kinase B (Akt) regulates the subcellular distribution and activity of the forkhead transcription factor AFX.

Authors:  A M Brownawell; G J Kops; I G Macara; B M Burgering
Journal:  Mol Cell Biol       Date:  2001-05       Impact factor: 4.272

6.  Structure and promoter characterization of the gene encoding the large subunit (R1 protein) of mouse ribonucleotide reductase.

Authors:  S Björklund; K Hjortsberg; E Johansson; L Thelander
Journal:  Proc Natl Acad Sci U S A       Date:  1993-12-01       Impact factor: 11.205

7.  Heterogeneous nuclear ribonucleoprotein (hnRNP) binding to hormone response elements: a cause of vitamin D resistance.

Authors:  Hong Chen; Martin Hewison; Bing Hu; John S Adams
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-25       Impact factor: 11.205

8.  Cytoplasmic sequestration of an O6-methylguanine-DNA methyltransferase enhancer binding protein in DNA repair-deficient human cells.

Authors:  F Y Chen; L C Harris; J S Remack; T P Brent
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-29       Impact factor: 11.205

9.  Identification of a 59 bp enhancer located at the first exon/intron boundary of the human O6-methylguanine DNA methyltransferase gene.

Authors:  L C Harris; J S Remack; T P Brent
Journal:  Nucleic Acids Res       Date:  1994-11-11       Impact factor: 16.971

10.  Evidence for a HeLa cell splicing activity that is necessary for activation of a regulated adenovirus 3' splice site.

Authors:  K Zerivitz; J P Kreivi; G Akusjärvi
Journal:  Nucleic Acids Res       Date:  1992-08-11       Impact factor: 16.971
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