OBJECTIVE: This study was designed to evaluate whether the high concentration of bilirubin is able to interfere the determination of alkaline phosphatase (ALP). METHODS: Clinical tests evaluating the interfering substance of bilirubin of various concentrations on the determination of ALP were conducted based upon the Clinical and Laboratory Standards Institute (CLSI) document EP7-A2, the most recent guideline on interference testing approved in 2005. RESULTS: Paired t-test comparing different doses of bilirubin revealed that the concentration of 1 000 μmmol/L bilirubin negatively interfered the determination of ALP levels. The experiment designed with five different concentrations of bilirubin showed that bilirubin can exert negative interference on the measurement of ALP in a linear pattern. CONCLUSION: High concentration of bilirubin can cause false measurement of ALP levels, probably interfering with the clinical prognosis of liver diseases.
OBJECTIVE: This study was designed to evaluate whether the high concentration of bilirubin is able to interfere the determination of alkaline phosphatase (ALP). METHODS: Clinical tests evaluating the interfering substance of bilirubin of various concentrations on the determination of ALP were conducted based upon the Clinical and Laboratory Standards Institute (CLSI) document EP7-A2, the most recent guideline on interference testing approved in 2005. RESULTS: Paired t-test comparing different doses of bilirubin revealed that the concentration of 1 000 μmmol/L bilirubin negatively interfered the determination of ALP levels. The experiment designed with five different concentrations of bilirubin showed that bilirubin can exert negative interference on the measurement of ALP in a linear pattern. CONCLUSION: High concentration of bilirubin can cause false measurement of ALP levels, probably interfering with the clinical prognosis of liver diseases.
Authors: Fatemeh Alaei Faradonbeh; Hana Lastuvkova; Jolana Cermanova; Milos Hroch; Zuzana Nova; Martin Uher; Petra Hirsova; Petr Pavek; Stanislav Micuda Journal: Front Physiol Date: 2022-03-21 Impact factor: 4.755