Siegfried Wagner1, Denise Maibaum1, Andreas Pich2, Ingo Nolte3, Hugo Murua Escobar4. 1. Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany. 2. Core Facility Proteomics, Hannover Medical School, Hannover, Germany. 3. Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany ingo.nolte@tiho-hannover.de. 4. Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany Division of Medicine, Department of Haematology/Oncology, University of Rostock, Rostock, Germany.
Abstract
BACKGROUND: Canine prostate cancer (PC) is a highly aggressive malignancy. However, in contrast to man, neither standard screening strategies nor curative therapeutic options exist for the companion animal. A prostate-specific membrane antigen (PSMA) screening as molecular marker akin to human PC is currently not available for dogs as data on specific canine PSMA detection are contradictory. MATERIALS AND METHODS: To evaluate an antibody for specific canine PSMA detection by western blotting (WB), lysates of three canine prostatic cell lines (CT1258, DT08/40, DT08/46) were comparatively analyzed by WB and mass spectrometry (MS) to the human cell lines VCaP, LnCaP and PC-3. RESULTS: MS analyses of the detected canine proteins confirmed cross reactivity of the antibody clone YPSMA-1 with canine PSMA. CONCLUSION: The MS analyses of the extracted canine protein bands proved that the YPSMA-1 clone is as well specific for canine PSMA in WB and, thus, represents a reliable tool for comparative PSMA studies. Copyright
BACKGROUND:Canineprostate cancer (PC) is a highly aggressive malignancy. However, in contrast to man, neither standard screening strategies nor curative therapeutic options exist for the companion animal. A prostate-specific membrane antigen (PSMA) screening as molecular marker akin to human PC is currently not available for dogs as data on specific caninePSMA detection are contradictory. MATERIALS AND METHODS: To evaluate an antibody for specific caninePSMA detection by western blotting (WB), lysates of three canine prostatic cell lines (CT1258, DT08/40, DT08/46) were comparatively analyzed by WB and mass spectrometry (MS) to the human cell lines VCaP, LnCaP and PC-3. RESULTS: MS analyses of the detected canine proteins confirmed cross reactivity of the antibody clone YPSMA-1 with caninePSMA. CONCLUSION: The MS analyses of the extracted canine protein bands proved that the YPSMA-1 clone is as well specific for caninePSMA in WB and, thus, represents a reliable tool for comparative PSMA studies. Copyright
Authors: Matthew Dowling; Jonathan Samuelson; Bahaa Fadl-Alla; Holly C Pondenis; Mark Byrum; Anne M Barger; Timothy M Fan Journal: PLoS One Date: 2019-01-02 Impact factor: 3.240