BACKGROUND: Although the imbalance of cytokines in Head and Neck Squamous Cell Carcinoma (HNSCC) is well known, there is scarce data regarding its occurrence during dysplasia, before the malignant transformation. OBJECTIVE: To determine whether laryngeal dysplasia patients show a different cytokine profile than patients with cancer and healthy controls. METHODS: Seventeen newly diagnosed, untreated larynx squamous cell carcinoma (SCC) and six laryngeal dysplasia patients as well as 22 healthy controls were analyzed for circulating cytokines. A flowcytometry Th1/Th2 cytokine array kit was used to quantitatively measure Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor-α (TNF-α) and Interferon-γ (IFN-γ) levels. Additionally, IL-8 levels were determined through ELISA. RESULTS: IL-6, IL-8 and IL-10 were determined to be statistically increased in SCC patients (p<0.05). IL-8 and IL-10 levels were also higher in SCC patients than dysplasia patients (p<0.05). Additionally, IL-6 and IL-10 were all found to be markedly increased in dysplasia patients compared with controls (p<0.05). CONCLUSION: Our results demonstrate an imbalance of IL-6 and IL-10 not only in HNSCC but also in laryngeal dysplasia.
BACKGROUND: Although the imbalance of cytokines in Head and Neck Squamous Cell Carcinoma (HNSCC) is well known, there is scarce data regarding its occurrence during dysplasia, before the malignant transformation. OBJECTIVE: To determine whether laryngeal dysplasiapatients show a different cytokine profile than patients with cancer and healthy controls. METHODS: Seventeen newly diagnosed, untreated larynx squamous cell carcinoma (SCC) and six laryngeal dysplasiapatients as well as 22 healthy controls were analyzed for circulating cytokines. A flowcytometry Th1/Th2 cytokine array kit was used to quantitatively measure Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor-α (TNF-α) and Interferon-γ (IFN-γ) levels. Additionally, IL-8 levels were determined through ELISA. RESULTS:IL-6, IL-8 and IL-10 were determined to be statistically increased in SCC patients (p<0.05). IL-8 and IL-10 levels were also higher in SCC patients than dysplasiapatients (p<0.05). Additionally, IL-6 and IL-10 were all found to be markedly increased in dysplasiapatients compared with controls (p<0.05). CONCLUSION: Our results demonstrate an imbalance of IL-6 and IL-10 not only in HNSCC but also in laryngeal dysplasia.
Authors: Oleksandr Ekshyyan; Alok R Khandelwal; Xiaohua Rong; Tara Moore-Medlin; Xiaohui Ma; Jonathan Steven Alexander; Cherie-Ann O Nathan Journal: Am J Transl Res Date: 2016-11-15 Impact factor: 4.060
Authors: Marcell Costa de Medeiros; Rajat Banerjee; Min Liu; Giovana Anovazzi; Nisha J D'Silva; Carlos Rossa Junior Journal: Oncotarget Date: 2017-06-15