Literature DB >> 2554860

Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains.

G Gerna1, A Sarasini, M Torsellini, A di Matteo, F Baldanti, M Parea, M Battaglia.   

Abstract

Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.

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Year:  1989        PMID: 2554860     DOI: 10.1007/BF01317927

Source DB:  PubMed          Journal:  Arch Virol        ISSN: 0304-8608            Impact factor:   2.574


  23 in total

1.  Epidemiology of human rotavirus Types 1 and 2 as studied by enzyme-linked immunosorbent assay.

Authors:  R H Yolken; R G Wyatt; G Zissis; C D Brandt; W J Rodriguez; H W Kim; R H Parrott; J J Urrutia; L Mata; H B Greenberg; A Z Kapikian; R M Chanock
Journal:  N Engl J Med       Date:  1978-11-23       Impact factor: 91.245

2.  Comparative sensitivities of solid-phase immune electron microscopy and enzyme-linked immunosorbent assay for serotyping of human rotavirus strains with neutralizing monoclonal antibodies.

Authors:  G Gerna; A Sarasini; B S Coulson; M Parea; M Torsellini; E Arbustini; M Battaglia
Journal:  J Clin Microbiol       Date:  1988-07       Impact factor: 5.948

3.  Production and characterization of neutralizing monoclonal antibody to a human rotavirus strain with a "super-short" RNA pattern.

Authors:  J M Tursi; M J Albert; R F Bishop
Journal:  J Clin Microbiol       Date:  1987-12       Impact factor: 5.948

4.  A candidate for a new serotype of human rotavirus.

Authors:  S Matsuno; A Hasegawa; A Mukoyama; S Inouye
Journal:  J Virol       Date:  1985-05       Impact factor: 5.103

5.  Rotavirus isolate WI61 representing a presumptive new human serotype.

Authors:  H F Clark; Y Hoshino; L M Bell; J Groff; G Hess; P Bachman; P A Offit
Journal:  J Clin Microbiol       Date:  1987-09       Impact factor: 5.948

6.  The outer capsid glycoprotein VP7 of simian rotavirus SA11 contains two distinct neutralization epitopes.

Authors:  G Gerna; A Sarasini; A di Matteo; N Passarani; V Gagliardi; G Milanesi; G C Astaldi Ricotti; M Battaglia
Journal:  J Gen Virol       Date:  1988-04       Impact factor: 3.891

7.  Detection of rotavirus in stool specimens with monoclonal and polyclonal antibody-based assay systems.

Authors:  C V Knisley; A J Bednarz-Prashad; L K Pickering
Journal:  J Clin Microbiol       Date:  1986-05       Impact factor: 5.948

8.  Detection of human rotaviruses which do not react with subgroup I- and II-specific monoclonal antibodies.

Authors:  L Svensson; L Grahnquist; C A Pettersson; M Grandien; G Stintzing; H B Greenberg
Journal:  J Clin Microbiol       Date:  1988-06       Impact factor: 5.948

9.  Production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzyme-linked immunosorbent assay for subgroup determination.

Authors:  K Taniguchi; T Urasawa; S Urasawa; T Yasuhara
Journal:  J Med Virol       Date:  1984       Impact factor: 2.327

10.  Synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system.

Authors:  M K Estes; S E Crawford; M E Penaranda; B L Petrie; J W Burns; W K Chan; B Ericson; G E Smith; M D Summers
Journal:  J Virol       Date:  1987-05       Impact factor: 5.103

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  2 in total

1.  Isolation in Europe of 69 M-like (serotype 8) human rotavirus strains with either subgroup I or II specificity and a long RNA electropherotype.

Authors:  G Gerna; A Sarasini; L Zentilin; A Di Matteo; P Miranda; M Parea; M Battaglia; G Milanesi
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

2.  Isolation and characterization of two distinct human rotavirus strains with G6 specificity.

Authors:  G Gerna; A Sarasini; M Parea; S Arista; P Miranda; H Brüssow; Y Hoshino; J Flores
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

  2 in total

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