BACKGROUND: Appropriate animal models are required to test novel therapeutics for head and neck squamous cell carcinoma (HNSCC) such as near-infrared (NIR) imaging-guided surgery. METHODS: We developed an optimized animal model of orthotopic HNSCC (in female athymic NMRI (Naval Medical Research Institute) nude mice) with a prolonged survival time. Resection of the orthotopic tumors was performed 30 days after implantation with or without the aid of a miniaturized clinical grade NIR optical imaging device, after systemic administration of a fluorescent RGD-based probe that targets αv β3 integrin. RESULTS: NIR optical imaging-guided surgery increased the recurrence-free survival rate by 50% through the detection of fluorescent cancer residues as small as 185 µm; these fragments could remain unidentified if resection was performed exclusively under unaided visual guidance. CONCLUSION: NIR optical imaging-guided surgery showed an improved HNSCC tumor resection quality in our optimized orthotopic animal model.
BACKGROUND: Appropriate animal models are required to test novel therapeutics for head and neck squamous cell carcinoma (HNSCC) such as near-infrared (NIR) imaging-guided surgery. METHODS: We developed an optimized animal model of orthotopic HNSCC (in female athymic NMRI (Naval Medical Research Institute) nude mice) with a prolonged survival time. Resection of the orthotopic tumors was performed 30 days after implantation with or without the aid of a miniaturized clinical grade NIR optical imaging device, after systemic administration of a fluorescent RGD-based probe that targets αv β3 integrin. RESULTS: NIR optical imaging-guided surgery increased the recurrence-free survival rate by 50% through the detection of fluorescent cancer residues as small as 185 µm; these fragments could remain unidentified if resection was performed exclusively under unaided visual guidance. CONCLUSION: NIR optical imaging-guided surgery showed an improved HNSCC tumor resection quality in our optimized orthotopic animal model.
Authors: Louise M Risør; Malene M Clausen; Zaza Ujmajuridze; Mohammed Farhadi; Kim F Andersen; Annika Loft; Jeppe Friborg; Andreas Kjaer Journal: J Nucl Med Date: 2021-12-02 Impact factor: 11.082
Authors: Anders Christensen; Katalin Kiss; Giedrius Lelkaitis; Karina Juhl; Morten Persson; Birgitte Wittenborg Charabi; Jann Mortensen; Julie Lyng Forman; Anne Lyngholm Sørensen; David Hebbelstrup Jensen; Andreas Kjaer; Christian von Buchwald Journal: BMC Cancer Date: 2017-08-25 Impact factor: 4.430
Authors: Hanadi Talal Ahmedah; Laurence H Patterson; Steven D Shnyder; Helen M Sheldrake Journal: Cancers (Basel) Date: 2017-05-26 Impact factor: 6.639
Authors: Ryan P Judy; Jane J Keating; Elizabeth M DeJesus; Jack X Jiang; Olugbenga T Okusanya; Shuming Nie; David E Holt; Sean P Arlauckas; Phillip S Low; E James Delikatny; Sunil Singhal Journal: Sci Rep Date: 2015-11-13 Impact factor: 4.379