Camile S Farah1,2, Andrew J Dalley1, Phan Nguyen3, Martin Batstone1,4, Farzaneh Kordbacheh1, Joanna Perry-Keene5, David Fielding6. 1. University of Queensland Centre for Clinical Research, The University of Queensland, Brisbane, Queensland, Australia. 2. Australian Centre for Oral Oncology Research and Education, Brisbane, Queensland, Australia. 3. Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide, Australia. 4. Maxillofacial Unit, Royal Brisbane and Women's Hospital, Brisbane, Queensland, Australia. 5. Department of Pathology, Royal Brisbane and Women's Hospital, Brisbane, Queensland, Australia. 6. Department of Thoracic Medicine, Royal Brisbane and Women's Hospital, Brisbane, Queensland, Australia.
Abstract
BACKGROUND: Incomplete primary tumor excision contributes to localized postsurgical recurrence of oral squamous cell carcinoma (OSCC). The purpose of this study was to provide molecular evidence that surgical margin definition using narrow band imaging (NBI) resulted in more complete OSCC excision than conventional white light (WL) panendoscopy. METHODS: Molecular divergence among tumor, WL, and NBI-defined surgical margins was compared in 18 patients through microarray analysis (GeneChip U133-plus-2.0). RESULTS: The numbers of differentially expressed genes (NBI = 4387; WL = 3266; vs tumor) signified that NBI placed margins into less involved tissue than WL examination. Principal component analysis segregated tumor, WL, and NBI tissues appropriately based solely on mRNA profiles, and unsupervised hierarchical clustering identified 4 patients (22%) who benefited directly from NBI surgical margin definition. Gene ontology enrichment indicated increasing cell phenotypic diversity: tumor<WL<NBI. CONCLUSION: Resection to NBI-defined margins will leave less dysplastic and malignant residual tissue and thereby increase ablative surgery success rates.
BACKGROUND: Incomplete primary tumor excision contributes to localized postsurgical recurrence of oral squamous cell carcinoma (OSCC). The purpose of this study was to provide molecular evidence that surgical margin definition using narrow band imaging (NBI) resulted in more complete OSCC excision than conventional white light (WL) panendoscopy. METHODS: Molecular divergence among tumor, WL, and NBI-defined surgical margins was compared in 18 patients through microarray analysis (GeneChip U133-plus-2.0). RESULTS: The numbers of differentially expressed genes (NBI = 4387; WL = 3266; vs tumor) signified that NBI placed margins into less involved tissue than WL examination. Principal component analysis segregated tumor, WL, and NBI tissues appropriately based solely on mRNA profiles, and unsupervised hierarchical clustering identified 4 patients (22%) who benefited directly from NBI surgical margin definition. Gene ontology enrichment indicated increasing cell phenotypic diversity: tumor<WL<NBI. CONCLUSION: Resection to NBI-defined margins will leave less dysplastic and malignant residual tissue and thereby increase ablative surgery success rates.
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