Zhengyu Li1, Jia Jia2, Jinhai Gou2, Xia Zhao3, Tao Yi4. 1. Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China. Electronic address: zhengyuli@scu.edu.cn. 2. Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China. 3. Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China. 4. Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China.
Abstract
OBJECTIVE: To determine the potential microRNA (miRNA) regulators of embryo implantation, as a continuation of genomic and proteomic research. DESIGN: Laboratory animal research. SETTING: University hospital laboratory. ANIMAL(S): Adult healthy female C57BL6/J mice (age 6-8 weeks, nonfertile, weighing 18-20 g each). INTERVENTION(S): Female mice were mated naturally with fertile males to produce pregnancy. Luminal epithelium was collected by laser-capture microdissection during the implantation period. Mouse models of pseudopregnancy, delayed implantation, and artificial decidualization were established. MAIN OUTCOME MEASURE(S): The miRNA profile in luminal epithelium was clarified by microarray analysis and validated by real-time reverse transcription polymerase chain reaction (qRT-PCR) in a series of models. Target genes were predicted and confirmed by luciferase activity assay. The role of miRNA in implantation was examined by loss-of-function and gain-of-function of miRNA in vitro and in vivo. RESULT(S): A total of 29 and 15 miRNAs were up- and down-regulated, respectively, during the implantation period; 11 of these miRNAs were validated by qRT-PCR. The profile of miR-451 was clarified in a series of models. A dual-luciferase activity assay showed that Ankrd46 was a target gene of miR-451. Loss-of-function by LV-miR-451 sponge or miR-451 inhibitor led to a reduced number of embryo implantations, but had little effect on fertilization. CONCLUSION(S): miR-451 was specifically up-regulated during the implantation period, and it may play a major role in embryo implantation by targeting Ankrd46.
OBJECTIVE: To determine the potential microRNA (miRNA) regulators of embryo implantation, as a continuation of genomic and proteomic research. DESIGN: Laboratory animal research. SETTING: University hospital laboratory. ANIMAL(S): Adult healthy female C57BL6/J mice (age 6-8 weeks, nonfertile, weighing 18-20 g each). INTERVENTION(S): Female mice were mated naturally with fertile males to produce pregnancy. Luminal epithelium was collected by laser-capture microdissection during the implantation period. Mouse models of pseudopregnancy, delayed implantation, and artificial decidualization were established. MAIN OUTCOME MEASURE(S): The miRNA profile in luminal epithelium was clarified by microarray analysis and validated by real-time reverse transcription polymerase chain reaction (qRT-PCR) in a series of models. Target genes were predicted and confirmed by luciferase activity assay. The role of miRNA in implantation was examined by loss-of-function and gain-of-function of miRNA in vitro and in vivo. RESULT(S): A total of 29 and 15 miRNAs were up- and down-regulated, respectively, during the implantation period; 11 of these miRNAs were validated by qRT-PCR. The profile of miR-451 was clarified in a series of models. A dual-luciferase activity assay showed that Ankrd46 was a target gene of miR-451. Loss-of-function by LV-miR-451 sponge or miR-451 inhibitor led to a reduced number of embryo implantations, but had little effect on fertilization. CONCLUSION(S): miR-451 was specifically up-regulated during the implantation period, and it may play a major role in embryo implantation by targeting Ankrd46.
Authors: N R Joshi; R W Su; G V R Chandramouli; S K Khoo; J W Jeong; S L Young; B A Lessey; A T Fazleabas Journal: Hum Reprod Date: 2015-09-14 Impact factor: 6.918