Literature DB >> 2554240

Transformation of NIH3T3 cells by A-MuLV proviral DNA: effect of plasmid linearization and carrier DNA on transformation efficiency.

S K Shore1, E P Reddy.   

Abstract

We have optimized the conditions for efficient NIH3T3 focus formation by calcium phosphate transfection of proviral Abelson-murine leukemia virus (A-MuLV) plasmid DNA. Linearized pA-MuLV, P120 or P160 strains, when transfected with calf thymus carrier DNA, will produce 40-50 foci/100 ng pA-MuLV without co-transfecting Moloney-murine leukemia virus (Mo-MuLV) plasmid DNA.

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Year:  1989        PMID： 2554240

Source DB:  PubMed          Journal:  Oncogene        ISSN： 0950-9232            Impact factor:   9.867


  2 in total

1.  Activation of murine c-abl protooncogene: effect of a point mutation on oncogenic activation.

Authors:  S K Shore; S L Bogart; E P Reddy
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

2.  Nuclear localization of v-Abl leads to complex formation with cyclic AMP response element (CRE)-binding protein and transactivation through CRE motifs.

Authors:  M C Birchenall-Roberts; F W Ruscetti; J J Kasper; D C Bertolette; Y D Yoo; O S Bang; M S Roberts; J M Turley; D K Ferris; S J Kim
Journal:  Mol Cell Biol       Date:  1995-11       Impact factor: 4.272
  2 in total

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