Arterial Stiffness by Aerobic Exercise Is Related with Aerobic Capacity, Physical Activity Energy Expenditure and Total Fat but not with Insulin Sensitivity in Obese Female Patients with Type 2 Diabetes.

BACKGROUND: Arterial stiffness is an important factor in atherosclerosis. Thus we examined whether aerobic exercise could reduce arterial stiffness in obese women with type 2 diabetes without diabetic complication.
METHODS: A total of 35 women with type 2 diabetes (body mass index, 26.6±2.8 kg/m(2); age, 56.4±1.9 years; duration of diabetes, 4.7±4.8 years) were assigned to aerobic exercise group (AEG) or control group (CG). AEG completed a 12-week exercise program (3.6 to 5.2 metabolic equivalents, 3 day/week, 60 min/day), with their exercise activities monitored by accelerometers. We measured abdominal total fat area (TFA), visceral fat area (VFA), and subcutaneous fat area (SFA) by computed tomography, insulin sensitivity by insulin tolerance test (KITT), and augmentation index (AIx) by SphygmoCor at baseline and at the end of the 12-week program.
RESULTS: The AIx was improved in the AEG compared with the CG (P<0.001). The percent change of AIx had significant correlation with the improvement of physical activity energy expenditure (PAEE), aerobic capacity, TFA, and SFA (r=-0.416, P=0.013; r=0.560, P<0.001; r=0.489, P=0.003; r=0.531, P=0.001, respectively), but not with insulin sensitivity, energy intake, or VFA.
CONCLUSION: Improvement in aortic stiffness by aerobic exercise is related with the improvement of aerobic capacity, PAEE, and total fat but not with insulin sensitivity or energy intake in obese women with type 2 diabetes.

RCT Entities:   Population Interventions Outcomes

INTRODUCTION

Diabetes is major global public health concern that affects 347 million people have diabetes worldwide [1], which is correlated with increasing cardiovascular disease that is responsible for 52% deaths in type 2 diabetes [2]. In addition, people with type diabetes are also at a 2 to 4 times higher risk of developing cardiovascular diseases compared with the normal population [3]. The highest prevalence of cardiovascular disease occurred among people with diabetes when hyperglycemia is accompanied by other risk factors such as hypertension, dyslipidemia, hyperinsulinemia, and obesity [4]. Especially, obesity is an independent risk factor for cardiovascular diseases [5]. However, reducing weight in obese patients with type 2 diabetes has been shown to be effective in minimizing cardiovascular disease-related deaths [5]. Therefore, losing weight in obese patients with type 2 diabetes will be effective in preventing cardiovascular diseases. In patients with type 2 diabetes, increased arterial stiffness is regarded as an early stage of arteriosclerosis and it is also regarded as a risk factor and prognostic marker for cardiovascular diseases [6]. Recently, a method of measuring augmentation index (AIx) and central aortic pressure noninvasively by analyzing the pulse waves of peripheral artery was introduced [7], and a large randomized study reported that AIx and central aortic pressure are associated with the development of cardiovascular diseases [8].

It is well known that adequate exercise, regardless of any changes in weight, independently improves cardiac function and arterial compliance, either preventing or stopping the progression of coronary artery diseases [9]. Kingwell et al. [10] compared the pulse wave velocity (PWV) of athletes and normal people and found that regular and constant exercise decreases PWV, and DeSouza et al. [11] showed that healthy adults who participated in moderate aerobic exercises for 2 to 3 months had improved arterial intima-media thickness and arterial compliance. The subjects of previous studies regarding arterial compliance only included normal population or patients with hypertension. However arterial stiffness may increase not only by hypertension but also by other risk factors of cardiovascular diseases such as old age, obesity, diabetes, and dyslipidemia. Furthermore, research regarding the correlation between reduced abdominal fat from exercises and improved arterial compliance in diabetic patients is still lacking.

Therefore, the aims of this study were to (1) examine whether aerobic exercise can alleviate arterial stiffness in patients with type 2 diabetes; (2) identify the correlation between the changes in arterial stiffness and physical activity level, aerobic capacity, abdominal fat, and insulin resistance.

METHODS

Study subjects

A total of 35 type 2 diabetes patients in the Diabetes Center at Eulji Hospital were enrolled from March to July in 2011. Subjects that were 45 to 65 years old and overweight with a body mass index (BMI) over 23 kg/m2 or obese female patients with hemoglobin A1c (HbA1c) levels less than 10% were included in this study. At baseline, 24, 10, and 1 of the 35 patients were under exercise and diet, oral hypoglycemic agent, and insulin treatments, respectively. The exclusion criteria were: type 1 diabetes patients, congestive heart failure, uncontrollable arrhythmia, severe valvular disease, patients with malignant tumor, patients under renal replacement therapy, patients that were difficult to perform tracking observations, patients refusing to be investigated, smokers, and patients who changed the medicine that they were taking. The Institutional Review Board of the Clinical Research Institute at Eulji Hospital approved the study protocol and informed consent was obtained from each subject.

Study design

During the 2 weeks of screening period, we requested that participants maintain their daily routine physical activity and dietary intake before beginning of the study. Two weeks later, the subjects were randomized into aerobic exercise group (AEG) or control group (CG). AEG group was performed walking exercise 3 day/week for 60 min/day at an exercise intensity of higher than 4 according to an accelerometer, corresponding to 3 to 5 metabolic equivalents (METs) [8] for 12 weeks, which corresponds to an energy expenditure of approximately 500 kcal/day. During 12 weeks, the subjects were monitored every 2 weeks for accurate measurements of their compliance, energy intake (EI), and physical activity energy expenditure (PAEE).

Measured categories and methods

Anthropometry and biochemical tests

The subjects' height, weight, and waist circumference were measured at baseline and after the 12-week period. The height and weight were measured when subjects were only wearing light clothing, and BMI was calculated by dividing weight (kg) by the square of height (m2). Waist circumference was measured between the lower ribs and iliac crest after exhale with a tape measure, and blood pressure was measured after 10 minutes of rest while sitting with a mercury sphygmomanometer (Yamasu, Tokyo, Japan).

As for biochemical tests, venous blood was collected 10 hours of fasting, which was centrifuged at 3,000 rpm for 15 minutes to separate the serum that was stored at -70℃ before being analyzed. Fasting glucose was measured by glucose oxidation method and HbA1c level was measured using high-performance liquid chromatography based on ion-exchange resin. Cholesterol, triglyceride, high density lipoprotein cholesterol, and low density lipoprotein cholesterol (LDL-C) were measured by enzyme-linked immunosorbent assay using an automatic chemistry analyzer (Hitachi 7170; Hitachi, Tokyo, Japan).

As for biochemical tests, venous blood was collected 10 hours of fasting, which was centrifuged at 3,000 rpm for 15 minutes to separate the serum that was stored at -70℃ before being analyzed. Fasting glucose was measured by glucose oxidation method and HbA1c level was measured using high-performance liquid chromatography based on ion-exchange resin. Cholesterol, triglyceride, high density lipoprotein cholesterol, and low density lipoprotein cholesterol (LDL-C) were measured by enzyme-linked immunosorbent assay using an automatic chemistry analyzer (Hitachi 7170; Hitachi, Tokyo, Japan).

Insulin resistance was calculated using insulin tolerance test (KITT) [12]. After 10 hours of fasting, an insulin tolerance test was performed. A 20-gauge catheter was inserted into a vein in one hand of the subject for blood collection and another catheter into the vein on the other side for insulin injection and glucose infusion at the end of the test. The appropriate insulin concentration (0.1 U per 1 kg) was calculated and prediluted insulin (Humulin R; Eli Lilly, Indianapolis, IN, USA) was injected into the antebrachial vein at resting state, while blood was collected from the vein in the opposite hand at baseline, 3, 6, 9, 12, and 15 minutes afterwards. One hundred milliliter of 20% glucose solution was infused 15 minutes after blood collection to prevent hypoglycemia, while the blood was immediately centrifuged to measure blood glucose levels. The glucose concentrations that were measured at different time points during insulin resistance test were entered into a computer software, where they were converted to each respective natural logarithm and the slope of regression line was measured using the values at 3 to 15 minutes. From this the half-life (t1/2) which is the time taken for baseline glucose level to drop by half was calculated and the rate constant for plasma glucose disappearance (KITT), the insulin resistance index, was obtained using the formula: KITT=0.693/(t1/2)×100 (%/min).

AIx was acquired by measuring arterial stiffness and pulse wave reflection using SphygmoCor system (SphygmoCoR; AtCor Medical Pty Ltd., Sydney, Austrailia). The pulse pressure (PP) waveform of peripheral artery was measured with applanation tonometer. All measurements were taken after at least 5 minutes of rest while sitting with either right radial artery for right-handed subjects or left transradial coronary artery for left-handed subjects. When 40 continuous waveforms were recorded, a validated transform function was used to obtain the PP waveform of aorta, and PWV was used as an evaluation index for aorta stiffness. At least two measurements per subject were performed. These measurements were checked whether the quality index provided by the software was over 80% and whether the variations between aorta PP waveforms were minimal by three medical doctors, after which only the valid measurements were included.

Definitions of major hemodynamic indices [13]: (1) Augmentation pressure (AP): maximum blood pressure (mm Hg) at the inflection point of systolic blood pressure; (2) AIx: AIx is this aortic AP expressed as a percentage of aortic PP: AIx=AP/PP×100 (%); (3) high AIx values denote severe aortic stiffness; (4) AIx adjusted to a hear rate of 75% (AIx@75): since AIx is affected by heart rate (HR), it is adjusted to a 'standard HR' of 75 bpm, the SphygomoCor Px software automatically decreases AIx by 4.8% per every 10 bpm rise in HR. This only applies to HR from 40 to 110 bpm.

Exercise intervention

The CG did not participate in any exercise programs, while AEG performed 60 minutes of walking exercise at an exercise intensity between 3.6 and 5.2 METs (Lifecorder activity levels 4 to 6) [14] three times per week to consume 500 kcal every day for 12 weeks. Both groups received one dietary education program at the beginning of the intervention.

Measurements of physical activity level and energy intake

To estimate the physical activity level of each subject, an accelerometer (Lifecorder; Suzuken Co., Nagoya, Japan) was used. The age, gender, height, weight, and measurement start date and time of each subject at baseline were entered. All subjects were requested to attach an accelerometer to their belt at all times except during sleep or shower during the intervention period and changes in weight were recorded every 2 weeks. Total energy expenditure (TEE), PAEE, and energy expenditure of each activity intensity levels were analyzed.

The subjects were instructed to fill out a 3-day meal record (twice during weekdays and once during weekend) and visited the hospital every 4 weeks to review their intake records and investigate their overall dietary intake. All subjects participated in a prior education program about how to fill out the meal record, where they were instructed to record all the food that they have eaten in one day and their ingredients for each meal, as well as any dietary supplements or snacks. To increase the accuracy of the records, professional dietitians used life-size food models (Korea Mirage Replica Inc., Incheon, Korea), measuring cups, measuring spoons, and estimated food size by eye measurements of food photos (The Korean Dietetic Association, 1999) to review the meal records with the subjects when the dietary questionnaires were collected. Meal record data were analyzed with a computer-aided nutritional analysis program (CAN-Pro version 2.0; Korean Nutrition Society, Seoul, Korea) to be converted to dietary intake.

Graded exercise test

The graded exercise test was performed using a stationary bicycle (ER 900, D-72475 Bits; JAGER, Wuerzburg, Germany), increasing the power by 20 W in every 2 minutes until the subjects could not continue exercising. Subjects' heart beats per minute, oxygen uptake, work done, and breathing rate were automatically recorded as numbers and graphs every 15 seconds, while blood pressure was recorded every 2 minutes by an automatic sphygmomanometer before, during, and after exercise. We measured anaerobic threshold using a Jaeger Oxycon Delta system.

Computed tomography

Computed tomography (GE, Milwaukee, WI, USA) of abdominal fat was measured based on methods used in previous studies, radiographic images were taken from the 4th to 5th lumber body to belly button with 10 mm above and below the range. Fat density was measured in Hounsfield unit in the range from -150 to -50 and was reconstructed. In other words, the total abdominal fat area within the abdominal cavity was calculated using a computer and the visceral fat area (VFA) was obtained by measuring the inner area with the abdomen and the dorsal peritoneum as the boundary. The subcutaneous fat area (SFA) was calculated by subtracting the total abdominal fat by VFA [15].

Statistical analysis

The mean and standard deviation for different variables in this study were performed using SPSS version 15.0 (SPSS Inc., Chicago, IL, USA). The statistical differences in physical characteristics, biochemical test results, abdominal fat, and insulin resistance of both CG and AEG before and after the 12-week period were determined using paired t-test, and the differences between the groups were determined using independent t-test and analysis of covariance, and finally bivariate correlation test was used. Statistical significance was set at P<0.05.

RESULTS

Baseline clinical characteristics

A total of 35 (CG=18, AEG=17) subjects were enrolled for this study. The average age was 56.5±3.4, average diabetes duration was 4.7±4.8 years, and average BMI was 26.6±1.9 kg/m2. Although there were no significant differences in age (P=0.072), diabetes duration (P=0.488), and BMI (P=0.071) between the two groups (Table 1), the waist circumference was higher in CG compared with AEG (P=0.006). Furthermore, the average HbA1c (P=0.986), KITT (P=0.628) which is the insulin resistance index, and AIx@75 (P=0.115) were similar in both groups (Table 1). There were no changes in drug intake of subjects in attempt to prevent hypoglycemia during the 12-week period. At baseline, 13 and 9 subjects, 3 and 7 subjects, and 1 and 0 subjects in AEG and CG were receiving exercise and diet, oral hypoglycemic agent, and insulin therapies, respectively, but there were no significant differences in receiving different types of therapy between the groups.

Table 1

Clinical characteristics at the baseline

Values are presented as mean±standard deviation. P values are obtained by independent t-test.

AEG, aerobic exercise group; CG, control group; BW, body weight; BMI, body mass index; WC, waist circumference; FPG, fasting plasma glucose; HbA1c, hemoglobin A1c; KITT, insulin sensitivity by insulin tolerance test; SBP, systolic blood pressure; DBP, diastolic blood pressure; TC, total cholesterol; TG, triglyceride; HDL-C, high density lipoprotein cholesterol; LDL-C, low density lipoprotein cholesterol; TFA, total fat area; VFA, visceral fat area; SFA, subcutaneous fat area; AT-VO2, anaerobic threshold-oxygen consumption; AIx@75, augmentation index 75%.

Changes in energy expenditure and energy intake

There were no significant difference in the TEE (P=0.741), PAEE (P=0.539), and EI (P=0.419) at baseline between groups. After 12 weeks, the TEE (P=0.042) and PAEE (P=0.001) of AEG increased from baseline, but no differences were observed in CG (P=0.651, P=0.572, respectively). After 12 weeks, the changes in TEE (P=0.027) and PAEE (P=0.001) were significantly higher in AEG compared with CG (Tables 2 and 3).

Table 2

Total and physical activity energy expenditure and dietary energy intake in each group at the baseline and during the 12 weeks intervention (kcal/day)

Values are presented as mean±standard deviation. P values are obtained by independent t-test.

AEG, aerobic exercise group; CG, control group; TEE, total energy expenditure; PAEE, physical activity energy expenditure; EI, energy intake.

aP<0.05 compared to baseline within group, bChange (percent change) was calculated as (12 weeks value-baseline value)×100/baseline value

Table 3

Changes in anthropometric, insulin sensitivity, regional fat, aerobic capacity, and AIx among two groups

Values are presented as mean±standard deviation. P values are obtained by independent t-test between groups.

AIx, augmentation index; AEG, aerobic exercise group; CG, control group; BW, body weight; BMI, body mass index; WC, waist circumference; TFA, total fat area; VFA, visceral fat area; SFA, subcutaneous fat area; AT-VO2, anaerobic threshold-oxygen consumption; KITT, insulin sensitivity by insulin tolerance test; AIx@75, augmentation index 75%.

a% Change (percent change) was calculated as (12 weeks value-baseline value)×100/baseline value, bP<0.05 significance from paired t-test within group.

In CG, EI decreased significantly from 1,956.9±176.8 to 1,730.1±284.9 kcal/day (P<0.001), and AEG also showed a significant decrease in EI, from 1,909.0±169.0 to 1,634.5±253.0 kcal/day (P<0.001), but the changes in EI were not significantly different between the two groups (Tables 2 and 3).

While change in AIx@75 was associated with changes in TEE (r=-0.376, P=0.026) and PAEE (r=-0.416, P=0.013), it was not correlated with the change in EI (Table 4).

Table 4

Factors associated with percent change of AIx@75 including all groups

AIx@75, augmentation index 75%; BW, body weight; BMI, body mass index; WC, waist circumference; TFA, total fat area; VFA, visceral fat area; SFA, subcutaneous fat area; FPG, fasting plasma glucose; HbA1c, hemoglobin A1c; KITT, insulin sensitivity by insulin tolerance test; TC, total cholesterol; TG, triglyceride; LDL-C, low density lipoprotein cholesterol; HDL-C, high density lipoprotein cholesterol; SBP, systolic blood pressure; DBP, diastolic blood pressure; TEE, total energy expenditure; PAEE, physical activity energy expenditure; EI, energy intake; AT-VO2, anaerobic threshold-oxygen consumption.

Changes in anthropometric and biochemical indices

After 12 weeks, both AEG and CG showed significant decreases in weight (P<0.001 and P=0.01, respectively), BMI (P<0.001 and P=0.02, respectively), and waist circumference (P<0.001 and P=0.04, respectively) from baseline (Table 3, Fig. 1).

Fig. 1

Change in body weight and abdominal fat after 3 months intervention among two groups. BMI, body mass index; TFA, total fat area; VFA, visceral fat area; SFA, subcutaneous fat area; CG, control group; AEG, aerobic exercise group. aP<0.05 from independent t-test.

After 12 weeks, the decreases in weight, BMI, and waist circumference were significantly were greater in AEG compared with CG (P=0.039, P=0.039, and P=0.020, respectively), and the decreases in triglyceride level was also greater in AEG than in CG (P=0.034). However the change in insulin resistance was not significantly different between two groups (Table 3, Fig. 1).

Although the change in AIx@75 was significantly correlated with change in waist circumference (r=0.348, P=0.044), it was not associated with insulin resistance improvement significantly (Table 4).

Changes in abdominal fat

After 12 weeks intervention, AEG showed significant decreases in total fat area (TFA; P<0.001), VFA (P<0.001), and SFA (P<0.001) from baseline. On the other hand, CG showed no significant differences in TFA (P=0.103) or VFA (P=0.356), but their SFA decreased significantly (P=0.047) from baseline. After 12 weeks, the changes in TFA (P=0.010), VFA (P=0.017), and SFA (P=0.044) decreased significantly in AEG compared with CG (Table 3, Fig. 1).

Although the change in AIx@75 was significantly related to the changes in SFA (r=0.531, P=0.001) and TFA (r=0.489, P=0.003), it was not associated with VFA (Table 4).

Changes in aerobic capacity and AIx

After 12 weeks, the aerobic capacity significantly increased in AEG from baseline (P<0.001), while CG did not show any differences, and the change in aerobic capacity increased significantly in AEG compared with CG (P<0.001). The differences in aerobic capacity between the two groups was still significant even after adjusting for subjects' baseline age, weight, waist circumference, total cholesterol, LDL-C, and aerobic capacity (P=0.002) (Table 3, Fig. 2).

Fig. 2

Change in augmentation index 75% (AIx@75), aerobic capacity and physical activity energy expenditure (PAEE) after 3 months intervention among two groups. AEG, aerobic exercise group; CG, control group; AC, aerobic capacity. aP<0.05 from independent t-test.

After 12 weeks, the aortic AIx@75 decreased significantly in AEG from baseline (P<0.001), while it was increased in CG from baseline (P<0.001). The difference in changes of aortic AIx@75 of AEG and CG after 12 weeks was significant (P<0.001) (Table 3), and this significance was maintained even after adjusting for age, weight, waist circumference, total cholesterol, and LDL-C (P<0.001).

The change in aortic AIx@75 had a significant correlation with the change in cardiorespiratory fitness (r=-0.560, P<0.001) (Table 4, Fig. 3).

Fig. 3

Correlation between physical activity energy expenditure (PAEE), aerobic capacity (AC), and augmentation index 75% (AIx@75).

DISCUSSION

Increased arterial stiffness gives rise to left ventricular overload and increases the tension of blood vessel wall, consequently leading to increased risks of cardiovascular diseases. Arterial stiffness can be change after exercise. Indeed, Lee et al. [16] showed that an acute treadmill exercise (60% HRmax) in patients with hypertension can lead to decreased blood pressure and improved vascular compliance. Furthermore, improvements of vascular compliance was greater in the 12 weeks compared with at the 6 weeks among subjects with hypertension taking antihypertensive drugs, which indicated prolonged aerobic exercise showed much effectiveness although temporary aerobic exercise does improve vascular compliance [17]. Also, Mustata et al. [18] reported that aerobic exercise 60 min/day for 3 day/week for 12 weeks had an effect of significantly decreasing arterial stiffness in patients with predialysis chronic kidney disease. In line with previous studies, this study showed AIx was significantly decreased in AEG compared with CG. The possible reason for the restoration of vascular compliance after exercise is the augmented blood flow and blood pressure during exercise helps maintain the normal functions of aortic endothelium and efficiently supplies blood to arteriole [19]. In addition, the increased production of nitric oxide in vascular endothelium with exercise might be possible reason for the benefits of exercise-induced decrease in arterial stiffness [20].

In general, arterial stiffness can be affected by age, obesity, hypertension, and diabetes, which are all risk factors for cardiovascular diseases [21]. Arterial stiffness has also shown to be correlated with insulin resistance because increased insulin resistance has been known to induce arterial stiffness through various mechanisms such as accelerated sympathetic nervous system, increased sodium uptake in kidneys, impaired vascular dilation, and accelerated renin-angiotensin-aldosterone system. These have been known to show significant correlation with AIx and fasting blood glucose [22], and Wilkinson et al. [23] showed that AIx is increased in diabetes. However, no differences in changes of fasting blood glucose, HbA1c, and KITT were observed between AEG and CG in this study, and no correlation between these variables and arterial stiffness was observed. A previous study reported that intense exercise is required to increase insulin sensitivity [24], while long-term period of exercise is required for moderate intense exercise [25,26]. Furthermore, Mustata et al. [18] reported that only 12 weeks of aerobic exercise is not sufficient to increase homeostasis model assessment of insulin resistance (HOMA-IR), and many other factors are involved in increasing HOMA-IR. However this study did not have enough exercise intensity, amount, and duration to increase insulin sensitivity, which might be a reason that changes in insulin sensitivity had no correlation with changes in AIx in this study. Further study that studies the association between enhanced insulin sensitivity and arterial stiffness with varying exercise intensity and duration is warranted.

Arterial stiffness and abdominal fat are known to show significant correlation. An aquarobics exercise for 60 min/day for 3 day/week for 8 weeks among middle-aged obese women reduced body fat and increased vascular compliance [27], while Kim et al. [28] showed a significant relationship between arterial stiffness measured by PWV and waist circumference but not with weight and BMI. In line, this study also showed a correlation between changes in AIx and changes in waist circumference but not with weight and BMI. As such, weight and BMI has, until now, failed to show any consistent correlation with long-term arterial calcification and arterial stiffness because BMI does not only reflect body fat but also muscle mass [29]; therefore, the association between BMI and arterial stiffness can differ depending on the ratio of fat and muscle. Furthermore, we found that AEG had significantly higher reductions in total abdominal fat, visceral fat, and subcutaneous fat compared with CG, of which changes in total abdominal fat and subcutaneous fat showed significant correlation with changes in AIx, suggesting that abdominal fat has larger implications on AIx than weight. Although changes in visceral fat did not such a significant correlation, its P value was 0.059, which is very close to being significant, therefore further study with a larger cohort and longer duration may be required to confirm this results.

Most studies regarding exercise reported that exercise alone does not have a large effect on weight reduction [30,31], but here we showed not only the significant reduction in weight of AEG (-3.1 kg) but also a significant reduction in subcutaneous fat of CG, unlike the previous studies. We speculate that the reason for such controversy is because both groups had reduced food intake at the end of study compared with baseline. However there was no difference in calorie intake in both groups. Arterial stiffness has been shown to be associated with increased aerobic exercise capacity through regular aerobic exercise [32]. Yoon et al. [33] showed that carotid-femoral artery PWV was negatively associated with VO2max which is an index of cardiorespiratory fitness among old-aged women (r=-0.533). Mustata et al. [18] also showed a significant association between the increased VO2max and AIx after 12 weeks of aerobic exercise with 40% to 60% intensity. This study also showed that AEG had increased aerobic capacity compared with CG, and the enhanced cardiorespiratory fitness showed a negative correlation with reduced arterial stiffness (r=-0.560), similarly to previous studies, and this had the highest correlation among other factors. As such, arterial compliance enhancement after exercise is known to be due to the increased aortic compliance, reduced peripheral blood vessel resistance, restoration of compliance, changes in plasma volume, and changes in endocrine system [34,35]. Aerobic exercise capacity is a strong predictor of total mortality due to cardiovascular diseases [36], and although this is greatly reduced in patients with type 2 diabetes [37], regular exercise can restore cardiorespiratory fitness and reduce risk factors for cardiovascular diseases. The American Diabetes Association particularly emphasizes the advantages of regular physical activities in diabetes prevention and treatment and recommends more than 30 minutes of moderate exercise every day [38]. They are also recommending more than 150 minutes of moderate exercise (50% to 70% of HRmax) every week and more than three times per week of aerobic exercise, especially to control blood glucose levels, maintain weight, and reduce risks of cardiovascular diseases [39]. In addition, this study showed that changes in AIx does not have a significant relationship with changes in calorie intake but does have a negative correlation with TEE and PAEE.

The limitations of this study were that the cohort size was small and that this was only a preliminary study. Also, this study does not provide any clues regarding how exercise intensity, amount, and duration can affect weight reduction and insulin resistance. Therefore further study with a larger cohort with differing exercise intensity, amount, and duration is needed.

In conclusion, 12 weeks of moderate aerobic exercise can reduce weight and abdominal fat, alleviate arterial stiffness, and improves aerobic capacity but does not decrease insulin resistance. In addition, changes in arterial stiffness was more associated with waist circumference and changes in abdominal fat rather than weight, and with changes in PAEE rather than changes in EI. These suggested that moderate intensity exercise improved arterial stiffness with relation to cardiorespiratory fitness and also with central obesity in type 2 diabetes.