Literature DB >> 25541363

Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the Vitek® MS system.

Jussimara Monteiro1, Fernanda Matsiko Inoue2, Ana Paula T Lobo2, Eduardo K Sugawara2, Fabiana M Boaretti2, Sergio Tufik2.   

Abstract

A simple and reliable protocol using 0.1% trifluoroacetic acid (TFA) was developed to identify bacteria directly from blood cultures on the Vitek® MS system. Results presented a correlation of 99% for Gram-negative bacteria at species level, and 86.3% and 82.3% of Gram-positive bacteria at the genus and species levels, respectively.
Copyright © 2014 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Bacterial identification; Blood culture; MALDI-TOF; Mass spectrometry; Trifluoroacetic acid; Vitek MS

Mesh：

Substances：

Year:  2014        PMID： 25541363     DOI： 10.1016/j.mimet.2014.12.009

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  11 in total

1.  Performance of microbial identification by MALDI-TOF MS and susceptibility testing by VITEK 2 from positive blood cultures after minimal incubation on solid media.

Authors:  S L Mitchell; K Alby
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2017-06-28       Impact factor: 3.267

2.  Rapid Identification of Microorganisms from Positive Blood Culture by MALDI-TOF MS After Short-Term Incubation on Solid Medium.

Authors:  Antonio Curtoni; Raffaella Cipriani; Elisa Simona Marra; Anna Maria Barbui; Rossana Cavallo; Cristina Costa
Journal:  Curr Microbiol       Date:  2016-11-17       Impact factor: 2.188

3.  Rapid identification of Gram-negative pathogens from positive blood cultures using MALDI-TOF MS following short-term incubation on solid media.

Authors:  Ioannis K Neonakis; Demetrios A Spandidos
Journal:  Biomed Rep       Date:  2022-04-08

Review 4.  Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Use with Positive Blood Cultures: Methodology, Performance, and Optimization.

Authors:  Matthew L Faron; Blake W Buchan; Nathan A Ledeboer
Journal:  J Clin Microbiol       Date:  2017-08-30       Impact factor: 5.948

5.  Same-Day Identification and Antimicrobial Susceptibility Testing of Bacteria in Positive Blood Culture Broths Using Short-Term Incubation on Solid Medium with the MicroFlex LT, Vitek-MS, and Vitek2 Systems.

Authors:  Jihye Ha; Sung Kuk Hong; Geum Hee Han; Myungsook Kim; Dongeun Yong; Kyungwon Lee
Journal:  Ann Lab Med       Date:  2018-05       Impact factor: 3.464

6.  Identification of Clinically Relevant Streptococcus and Enterococcus Species Based on Biochemical Methods and 16S rRNA, sodA, tuf, rpoB, and recA Gene Sequencing.

Authors:  Maja Kosecka-Strojek; Mariola Wolska; Dorota Żabicka; Ewa Sadowy; Jacek Międzobrodzki
Journal:  Pathogens       Date:  2020-11-11

7.  Evaluation of Rapid Sepsityper® protocol and specific MBT-Sepsityper module (Bruker Daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS.

Authors:  Léa Ponderand; Patricia Pavese; Danièle Maubon; Emmanuelle Giraudon; Thomas Girard; Caroline Landelle; Max Maurin; Yvan Caspar
Journal:  Ann Clin Microbiol Antimicrob       Date:  2020-12-09       Impact factor: 3.944

8.  Optimized identification of microorganisms directly from positive blood cultures by MALDI-TOF to improve antimicrobial treatment.

Authors:  P García Clemente; P Romero-Gómez; J García-Rodríguez; E Cendejas-Bueno
Journal:  Rev Esp Quimioter       Date:  2022-05-23       Impact factor: 2.515

9.  MALDI-TOF MS Versus VITEK®2: Comparison of Systems for the Identification of Microorganisms Responsible for Bacteremia.

Authors:  Filomena Febbraro; Donatella Maria Rodio; Gianluca Puggioni; Guido Antonelli; Valeria Pietropaolo; Maria Trancassini
Journal:  Curr Microbiol       Date:  2016-09-12       Impact factor: 2.188

10.  Direct Identification and Antimicrobial Susceptibility Testing of Bacteria From Positive Blood Culture Bottles by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and the Vitek 2 System.

Authors:  Sung Jin Jo; Kang Gyun Park; Kyungja Han; Dong Jin Park; Yeon-Joon Park
Journal:  Ann Lab Med       Date:  2016-03       Impact factor: 3.464
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