Lavinia N Arend1, Paula Toledo2, Marcelo Pilonetto3, Felipe F Tuon4. 1. Division of Infectious Diseases, Universidade Federal do Parana, Curitiba, PR, Brazil; Bacteriology Section, Laboratório Central de Saúde Pública do Estado Laboratorio Central-PR, Sao Jose do Pinhais, PR, Brazil. 2. Medicine Department, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil; Epidemiologic Surveillance Center, Secretaria Municipal de Saúde de Curitiba, Curitiba, PR, Brazil. 3. Division of Infectious Diseases, Universidade Federal do Parana, Curitiba, PR, Brazil; Microbiology Department, School of Health and Biosciences, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil. 4. Division of Infectious Diseases, Universidade Federal do Parana, Curitiba, PR, Brazil. Electronic address: flptuon@gmail.com.
Abstract
BACKGROUND: Klebsiella pneumoniae carbapenemase-producing K pneumoniae (KPC-KP) outbreaks have been reported in many countries, including Brazil. The incidence of KPC-KP infection has increased in the first semester of 2011 in Curitiba, the capital of Parana, in Southern Brazil.The aim of this study was to characterize the infections and clonal diversity of KPC-KP isolates from several institutions in Curitiba. METHODS: KPC-KP from several clinical samples and rectal swabs taken between April 2010 and July 2012 were included. One isolate per patient was evaluated. All isolates were submitted to polymerase chain reaction (PCR) for blaKPC. The genetic relatedness was evaluated using strain clustering by an automated repetitive extragenic palindromic (rep) PCR-based typing system. RESULTS: There were 641 samples that were positive for K pneumoniae carbapenemase-2 carbapenemase. There were 129 samples randomly selected for clonality evaluation. PCR and strain clustering by the automated rep PCR-based typing system identified 7 clones (A-C and E-H). Clone E was identified in only 1 hospital, and all other clones were found in >2 hospitals. Clones C and G were the most disseminated among hospitals. The infection and colonization occurred in 14 out of the 32 main hospitals in town. Similar clones were found in 2 hospitals that are administered by the same group. Another clone (H) was found in 2 hospitals receiving patients from the same municipal emergency unit. CONCLUSION: The KPC-KP outbreak in Curitiba is polyclonal, and the source is unknown. Some hospitals share the same clones.
BACKGROUND:Klebsiella pneumoniae carbapenemase-producing K pneumoniae (KPC-KP) outbreaks have been reported in many countries, including Brazil. The incidence of KPC-KP infection has increased in the first semester of 2011 in Curitiba, the capital of Parana, in Southern Brazil.The aim of this study was to characterize the infections and clonal diversity of KPC-KP isolates from several institutions in Curitiba. METHODS: KPC-KP from several clinical samples and rectal swabs taken between April 2010 and July 2012 were included. One isolate per patient was evaluated. All isolates were submitted to polymerase chain reaction (PCR) for blaKPC. The genetic relatedness was evaluated using strain clustering by an automated repetitive extragenic palindromic (rep) PCR-based typing system. RESULTS: There were 641 samples that were positive for K pneumoniae carbapenemase-2 carbapenemase. There were 129 samples randomly selected for clonality evaluation. PCR and strain clustering by the automated rep PCR-based typing system identified 7 clones (A-C and E-H). Clone E was identified in only 1 hospital, and all other clones were found in >2 hospitals. Clones C and G were the most disseminated among hospitals. The infection and colonization occurred in 14 out of the 32 main hospitals in town. Similar clones were found in 2 hospitals that are administered by the same group. Another clone (H) was found in 2 hospitals receiving patients from the same municipal emergency unit. CONCLUSION: The KPC-KP outbreak in Curitiba is polyclonal, and the source is unknown. Some hospitals share the same clones.
Authors: Jussara Kasuko Palmeiro; Robson Francisco de Souza; Marcos André Schörner; Hemanoel Passarelli-Araujo; Ana Laura Grazziotin; Newton Medeiros Vidal; Thiago Motta Venancio; Libera Maria Dalla-Costa Journal: Front Microbiol Date: 2019-07-23 Impact factor: 5.640
Authors: Marcelo Pillonetto; Regiane Tigulini de Souza Jordão; Gabriel Savogin Andraus; Ricardo Bergamo; Fabiano Barreto Rocha; Mayara Caroline Onishi; Bernardo Montesanti Machado de Almeida; Keite da Silva Nogueira; Amanda Dal Lin; Viviane Maria de Carvalho Hessel Dias; André Luiz de Abreu Journal: Front Public Health Date: 2021-01-14
Authors: V M de Carvalho Hessel Dias; F Tuon; P de Jesus Capelo; J P Telles; C M C B Fortaleza; C Pellegrino Baena Journal: J Hosp Infect Date: 2021-11-19 Impact factor: 3.926
Authors: Viviane Maria de Carvalho Hessel Dias; Daniela Maria Waszak da Silva; Marion Burger; Alcides Augusto Souto de Oliveira; Patrícia de Jesus Capelo; Fabio Augusto da Rocha Specian; Marianna Cavina de Figueiredo; Felipe Francisco Tuon; Cristina Pellegrino Baena Journal: Braz J Infect Dis Date: 2021-02-13 Impact factor: 3.257