Gábor Feigl1, Nóra Lehotai2, Árpád Molnár2, Attila Ördög2, Marta Rodríguez-Ruiz3, José M Palma3, Francisco J Corpas3, László Erdei2, Zsuzsanna Kolbert2. 1. Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary and fglgbr@gmail.com. 2. Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary and. 3. Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain.
Abstract
BACKGROUND AND AIMS: Zinc (Zn) is an essential micronutrient naturally present in soils, but anthropogenic activities can lead to accumulation in the environment and resulting damage to plants. Heavy metals such as Zn can induce oxidative stress and the generation of reactive oxygen and nitrogen species (ROS and RNS), which can reduce growth and yield in crop plants. This study assesses the interplay of these two families of molecules in order to evaluate the responses in roots of two Brassica species under high concentrations of Zn. METHODS: Nine-day-old hydroponically grown Brassica juncea (Indian mustard) and B. napus (oilseed rape) seedlings were treated with ZnSO4 (0, 50, 150 and 300 µm) for 7 d. Stress intensity was assessed through analyses of cell wall damage and cell viability. Biochemical and cellular techniques were used to measure key components of the metabolism of ROS and RNS including lipid peroxidation, enzymatic antioxidants, protein nitration and content of superoxide radical ([Formula: see text]), nitric oxide (NO) and peroxynitrite (ONOO(-)). KEY RESULTS: Analysis of morphological root damage and alterations of microelement homeostasis indicate that B. juncea is more tolerant to Zn stress than B. napus. ROS and RNS parameters suggest that the oxidative components are predominant compared with the nitrosative components in the root system of both species. CONCLUSIONS: The results indicate a clear relationship between ROS and RNS metabolism as a mechanism of response against stress caused by an excess of Zn. The oxidative stress components seem to be more dominant than the elements of the nitrosative stress in the root system of these two Brassica species.
BACKGROUND AND AIMS: Zinc (Zn) is an essential micronutrient naturally present in soils, but anthropogenic activities can lead to accumulation in the environment and resulting damage to plants. Heavy metals such as Zn can induce oxidative stress and the generation of reactive oxygen and nitrogen species (ROS and RNS), which can reduce growth and yield in crop plants. This study assesses the interplay of these two families of molecules in order to evaluate the responses in roots of two Brassica species under high concentrations of Zn. METHODS: Nine-day-old hydroponically grown Brassica juncea (Indian mustard) and B. napus (oilseed rape) seedlings were treated with ZnSO4 (0, 50, 150 and 300 µm) for 7 d. Stress intensity was assessed through analyses of cell wall damage and cell viability. Biochemical and cellular techniques were used to measure key components of the metabolism of ROS and RNS including lipid peroxidation, enzymatic antioxidants, protein nitration and content of superoxide radical ([Formula: see text]), nitric oxide (NO) and peroxynitrite (ONOO(-)). KEY RESULTS: Analysis of morphological root damage and alterations of microelement homeostasis indicate that B. juncea is more tolerant to Zn stress than B. napus. ROS and RNS parameters suggest that the oxidative components are predominant compared with the nitrosative components in the root system of both species. CONCLUSIONS: The results indicate a clear relationship between ROS and RNS metabolism as a mechanism of response against stress caused by an excess of Zn. The oxidative stress components seem to be more dominant than the elements of the nitrosative stress in the root system of these two Brassica species.
Authors: Filis Morina; Ljubinko Jovanović; Ljiljana Prokić; Sonja Veljović-Jovanović; J Andrew C Smith Journal: Environ Sci Pollut Res Int Date: 2016-02-11 Impact factor: 4.223
Authors: Ann Cuypers; Sophie Hendrix; Rafaela Amaral Dos Reis; Stefanie De Smet; Jana Deckers; Heidi Gielen; Marijke Jozefczak; Christophe Loix; Hanne Vercampt; Jaco Vangronsveld; Els Keunen Journal: Front Plant Sci Date: 2016-04-25 Impact factor: 5.753
Authors: Fernanda S Farnese; Paulo E Menezes-Silva; Grasielle S Gusman; Juraci A Oliveira Journal: Front Plant Sci Date: 2016-04-12 Impact factor: 5.753