Literature DB >> 25537838

The suspension culture of undifferentiated human pluripotent stem cells using spinner flasks.

Vincent C Chen1, Larry A Couture.   

Abstract

Suspension cell culture systems with superior scalability, controllability, and monitoring options are an attractive alternative to static adherent culture methods for expansion and production of human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent cells (iPSCs). In this chapter, we describe a scalable suspension culture system using serum-free, feeder-free, matrix-free, and defined culture conditions with spinner flasks for hPSC maintenance and expansion. This suspension culture system provides an efficient and GMP-compatible process for large-scale manufacture of hPSCs.

Entities:  

Mesh：

Year:  2015        PMID： 25537838     DOI： 10.1007/7651_2014_118

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

Review 1.  Pluripotent Stem Cell-Derived Cardiomyocytes as a Platform for Cell Therapy Applications: Progress and Hurdles for Clinical Translation.

Authors:  Angelos Oikonomopoulos; Tomoya Kitani; Joseph C Wu
Journal:  Mol Ther       Date:  2018-03-06       Impact factor: 11.454

Review 2.  Human pluripotent stem cells: Prospects and challenges as a source of cardiomyocytes for in vitro modeling and cell-based cardiac repair.

Authors:  Matthew E Hartman; Dao-Fu Dai; Michael A Laflamme
Journal:  Adv Drug Deliv Rev       Date:  2015-05-14       Impact factor: 15.470

3.  Perspectives on scaling production of adipose tissue for food applications.

Authors:  John S K Yuen; Andrew J Stout; N Stephanie Kawecki; Sophia M Letcher; Sophia K Theodossiou; Julian M Cohen; Brigid M Barrick; Michael K Saad; Natalie R Rubio; Jaymie A Pietropinto; Hailey DiCindio; Sabrina W Zhang; Amy C Rowat; David L Kaplan
Journal:  Biomaterials       Date:  2021-11-29       Impact factor: 15.304

4.  Quantification of Etoposide Hypersensitivity: A Sensitive, Functional Method for Assessing Pluripotent Stem Cell Quality.

Authors:  Frank J Secreto; Xing Li; Alyson J Smith; Elizabeth S Bruinsma; Ester Perales-Clemente; Saji Oommen; Gresin Hawse; Sybil C L Hrstka; Bonnie K Arendt; Emma B Brandt; Dennis A Wigle; Timothy J Nelson
Journal:  Stem Cells Transl Med       Date:  2017-09-19       Impact factor: 6.940

5.  Computational fluid dynamics modeling, a novel, and effective approach for developing scalable cell therapy manufacturing processes.

Authors:  Mehdi Shafa; Krishna M Panchalingam; Tylor Walsh; Thomas Richardson; Behnam Ahmadian Baghbaderani
Journal:  Biotechnol Bioeng       Date:  2019-09-23       Impact factor: 4.530

6.  Kinetic modeling of human induced pluripotent stem cell expansion in suspension culture.

Authors:  Vytautas Galvanauskas; Rimvydas Simutis; Suman Chandra Nath; Masahiro Kino-Oka
Journal:  Regen Ther       Date:  2019-04-25       Impact factor: 3.419

Review 7.  Systems for Muscle Cell Differentiation: From Bioengineering to Future Food.

Authors:  Kah-Yin Lee; Hui-Xin Loh; Andrew C A Wan
Journal:  Micromachines (Basel)       Date:  2021-12-31       Impact factor: 2.891
  7 in total

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