| Literature DB >> 25536094 |
Ramia Safar1, Carole Ronzani, Roudayna Diab, Jérôme Chevrier, Danièle Bensoussan, Stéphanie Grandemange, Alain Le Faou, Bertrand H Rihn, Olivier Joubert.
Abstract
S-Nitrosoglutathione (GSNO) is a good candidate for nitric oxide (NO(•)) delivery, and its nanoformulation improves NO(•) stability and bioavailability. We have compared the effect of empty Eudragit nanoparticles (eENP), GSNO-loaded ENP (gENP), and free GSNO on THP-1 human monocytic cell line. We investigated cellular viability and growth by WST-1 and trypan blue tests. ENP uptake was studied using transmission electron microscopy, confocal microscopy, and flow cytometry. Transcriptomic profiles were obtained using microarray. ENP entered cells by clathrin- and caveolae-mediated endocytosis. Exposure to either free GSNO or gENP induced an activation of genes from the same clusters, in favor of intracellular delivery of GSNO by ENP. GSNO nanoformulation might be a therapeutic option for NO(•) delivery.Entities:
Keywords: S-nitrosoglutathione; endocytosis; monocytes; nitric oxide; polymeric nanoparticles; toxicity; toxicogenomic; transcriptome; uptake
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Year: 2015 PMID： 25536094 DOI： 10.1021/mp5006382
Source DB: PubMed Journal: Mol Pharm ISSN： 1543-8384 Impact factor: 4.939