Lei Jing1, Yun Wang2, Xiao-Min Zhao3, Bing Zhao4, Ji-Ju Han2, Shu-Cun Qin2, Xue-Jun Sun5. 1. Key Laboratory of Atherosclerosis in Universities of Shandong (Taishan Medical University), Taian, 271000, China; Department of Pharmocology, Taishan Medical University, Taian, 271000, China; Department of Pharmacy, Dongping County People's Hospital, Dongping, 271500, China. 2. Key Laboratory of Atherosclerosis in Universities of Shandong (Taishan Medical University), Taian, 271000, China. 3. Key Laboratory of Atherosclerosis in Universities of Shandong (Taishan Medical University), Taian, 271000, China; Department of Pharmocology, Taishan Medical University, Taian, 271000, China. Electronic address: zhaoxiaominty@hotmail.com. 4. Key Laboratory of Atherosclerosis in Universities of Shandong (Taishan Medical University), Taian, 271000, China; Department of Pharmocology, Taishan Medical University, Taian, 271000, China; Department of Pharmacy, Boshan District Hospital of Zibo, Boshan, 255200, China. 5. Department of Diving Medicine, the Second Military Medical University, Shanghai, 200433, China.
Abstract
BACKGROUND: Infusion with hydrogen gas-saturated saline has recently been reported to exert antioxidant and anti-inflammatory activity that may protect against organ damage induced by oxidative stress. Therefore because oxidative stress plays a significant role in the pathophysiology of myocardial infarction (MI), the aim of our study was to investigate whether hydrogen-rich saline has cardioprotective effects against isoproterenol-induced MI in rats. METHODS: An acute MI model was induced in male Wistar rats by subcutaneous injection of isoproterenol. Different doses of hydrogen-rich saline (5, 7.5, and 10 mL/kg body weight i.p.) or Vitamin C (250 mg/kg body weight i.g.) were administered to the rats. Oxidative stress indices including levels of myocardial marker enzymes, inflammatory cytokines, membrane-bound myocardial enzymes and histopathological changes were measured. RESULTS: Compared with those in isoproterenol-MI group, hydrogen-rich saline decreased malondialdehyde and 8-hydroxy-desoxyguanosine concentrations, enhanced superoxide dismutase and Na(+)-K(+)-ATPase activity, lowered Ca(2+)-ATPase activity and decreased interleukin-6 and tumour necrosis factor-α levels in the serum and/or cardiac tissue of rats. Hydrogen-rich saline pretreatment also diminished infarct size, improved left heart function, and ameliorated pathological changes of the left heart. CONCLUSION: From these results, hydrogen-rich saline exerts cardiovascular protective effects against isoproterenol-induced MI at least in part via interactions which evoke antioxidant and anti-inflammatory activities.
BACKGROUND: Infusion with hydrogen gas-saturated saline has recently been reported to exert antioxidant and anti-inflammatory activity that may protect against organ damage induced by oxidative stress. Therefore because oxidative stress plays a significant role in the pathophysiology of myocardial infarction (MI), the aim of our study was to investigate whether hydrogen-rich saline has cardioprotective effects against isoproterenol-induced MI in rats. METHODS: An acute MI model was induced in male Wistar rats by subcutaneous injection of isoproterenol. Different doses of hydrogen-rich saline (5, 7.5, and 10 mL/kg body weight i.p.) or Vitamin C (250 mg/kg body weight i.g.) were administered to the rats. Oxidative stress indices including levels of myocardial marker enzymes, inflammatory cytokines, membrane-bound myocardial enzymes and histopathological changes were measured. RESULTS: Compared with those in isoproterenol-MI group, hydrogen-rich saline decreased malondialdehyde and 8-hydroxy-desoxyguanosine concentrations, enhanced superoxide dismutase and Na(+)-K(+)-ATPase activity, lowered Ca(2+)-ATPase activity and decreased interleukin-6 and tumour necrosis factor-α levels in the serum and/or cardiac tissue of rats. Hydrogen-rich saline pretreatment also diminished infarct size, improved left heart function, and ameliorated pathological changes of the left heart. CONCLUSION: From these results, hydrogen-rich saline exerts cardiovascular protective effects against isoproterenol-induced MI at least in part via interactions which evoke antioxidant and anti-inflammatory activities.