Julie H Laursen1, Helle Bach Søndergaard2, Anders Albrechtsen3, Ruth Frikke-Schmidt4, Nils Koch-Henriksen5, Per Soelberg Sørensen2, Finn Sellebjerg2, Annette Oturai2. 1. Department of Neurology, Rigshospitalet, Copenhagen University Hospital, Danish Multiple Sclerosis Center, Denmark juliehejgaard@hotmail.com. 2. Department of Neurology, Rigshospitalet, Copenhagen University Hospital, Danish Multiple Sclerosis Center, Denmark. 3. Centre of Bioinformatics, Faculty of Science, University of Copenhagen, Denmark. 4. Department of Clinical Biochemistry, Rigshospitalet, Copenhagen University Hospital, Denmark. 5. Rigshospitalet, Copenhagen University Hospital, Danish Multiple Sclerosis Treatment Register, Denmark/Clinical Institute, Department of Clinical Epidemiology, University of Aarhus, Denmark.
Abstract
BACKGROUND: Evidence is accumulating supporting a beneficial effect of vitamin D in multiple sclerosis (MS). Genome-wide association studies (GWAS) have shown significant associations between 25-hydroxyvitamin D (25(OH)D) and single nucleotide polymorphisms (SNPs) in key genes in the vitamin D metabolism. OBJECTIVE: To examine the association between 25(OH)D and six GWAS SNPs and environmental factors in 1497 MS patients. METHODS: Blood samples and lifestyle questionnaires were collected between 2009 and 2012. Genotyping of GC-, CYP2R1- and NADSYN1-SNPs was performed by TaqMan allelic discrimination (Life Technologies). RESULTS: We found significant associations between 25(OH)D and SNPs in GC (rs7041, p = 0.01 and rs2282679, p = 0.03) and CYP2R1 (rs10741657, p =1.8 × 10(-4)). Season of blood sampling (p = 2.8 × 10(-31)), sex (p = 1.9 × 10(-5)), BMI (p = 2.3 × 10(-5)), vitamin supplements (p = 7.0 × 10(-22)), and fish intake (p = 0.02) also had significant effects on 25(OH)D. CONCLUSION: In this cross-sectional study, we found significant effects of environmental factors and SNPs in GC and CYP2R1 on 25(OH)D in MS patients. Since 25(OH)D might have protective effects in MS, and vitamin D supply is a modifiable factor, it may be important to include this in the MS treatment regimen.
BACKGROUND: Evidence is accumulating supporting a beneficial effect of vitamin D in multiple sclerosis (MS). Genome-wide association studies (GWAS) have shown significant associations between 25-hydroxyvitamin D (25(OH)D) and single nucleotide polymorphisms (SNPs) in key genes in the vitamin D metabolism. OBJECTIVE: To examine the association between 25(OH)D and six GWAS SNPs and environmental factors in 1497 MS patients. METHODS: Blood samples and lifestyle questionnaires were collected between 2009 and 2012. Genotyping of GC-, CYP2R1- and NADSYN1-SNPs was performed by TaqMan allelic discrimination (Life Technologies). RESULTS: We found significant associations between 25(OH)D and SNPs in GC (rs7041, p = 0.01 and rs2282679, p = 0.03) and CYP2R1 (rs10741657, p =1.8 × 10(-4)). Season of blood sampling (p = 2.8 × 10(-31)), sex (p = 1.9 × 10(-5)), BMI (p = 2.3 × 10(-5)), vitamin supplements (p = 7.0 × 10(-22)), and fish intake (p = 0.02) also had significant effects on 25(OH)D. CONCLUSION: In this cross-sectional study, we found significant effects of environmental factors and SNPs in GC and CYP2R1 on 25(OH)D in MS patients. Since 25(OH)D might have protective effects in MS, and vitamin D supply is a modifiable factor, it may be important to include this in the MS treatment regimen.
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Authors: Brooke Rhead; Maria Bäärnhielm; Milena Gianfrancesco; Amanda Mok; Xiaorong Shao; Hong Quach; Ling Shen; Catherine Schaefer; Jenny Link; Alexandra Gyllenberg; Anna Karin Hedström; Tomas Olsson; Jan Hillert; Ingrid Kockum; M Maria Glymour; Lars Alfredsson; Lisa F Barcellos Journal: Neurol Genet Date: 2016-09-13