G Russmueller1, D Moser1, T Würger2, F Wrba2, P Christopoulos3, G Kostakis4, R Seemann1, V Stadler1, G Wimmer1, G Kornek5, A Psyrri6, M Filipits7, C Perisanidis1. 1. Department of Cranio-, Maxillofacial and Oral Surgery, Medical University of Vienna, Austria. 2. Department of Clinical Pathology, Medical University of Vienna, Austria. 3. Department of Maxillofacial and Oral Surgery, University of Athens, Greece. Electronic address: christos.perisanidis@meduniwien.ac.at. 4. Department of Maxillofacial and Oral Surgery, University of Athens, Greece. 5. Department of Medicine I, Medical University of Vienna, Austria. 6. Division of Oncology, Second Department of Internal Medicine, Attikon University Hospital, Athens, Greece. 7. Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, Austria.
Abstract
OBJECTIVES: We aimed to determine the prognostic significance of receptor activator of nuclear factor kappa-B ligand (RANKL), RANK and osteoprotegerin (OPG) in patients with oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: The protein expression of RANKL, RANK and OPG was assessed by immunohistochemistry on pretreatment biopsies of 93 patients with locally advanced OSCC who received preoperative chemoradiotherapy (CRT). The primary endpoint was cancer-specific survival. Secondary endpoints were correlation of biomarkers with bone invasion and pathological tumor response. Kaplan-Meier curves and Cox regression models were used for survival analyses. RESULTS: A significantly higher OPG expression was demonstrated in patients with malignant bone invasion and non-responders to CRT as compared to patients without bone invasion and responders (p=0.032 and p=0.033, respectively). Multivariate analysis revealed that higher OPG expression was independently associated with shorter cancer-specific survival (p=0.04). The expression status of RANKL and RANK was not significantly related to clinicopathological characteristics and had no impact on survival of OSCC patients. CONCLUSION: Upregulation of OPG expression is associated with bone invasion, poor pathological tumor regression to neoadjuvant CRT, and worse long-term cancer-specific survival in patients with locally advanced OSCC. Our results indicate that OPG may be a novel prognostic biomarker in oral cancer.
OBJECTIVES: We aimed to determine the prognostic significance of receptor activator of nuclear factor kappa-B ligand (RANKL), RANK and osteoprotegerin (OPG) in patients with oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: The protein expression of RANKL, RANK and OPG was assessed by immunohistochemistry on pretreatment biopsies of 93 patients with locally advanced OSCC who received preoperative chemoradiotherapy (CRT). The primary endpoint was cancer-specific survival. Secondary endpoints were correlation of biomarkers with bone invasion and pathological tumor response. Kaplan-Meier curves and Cox regression models were used for survival analyses. RESULTS: A significantly higher OPG expression was demonstrated in patients with malignant bone invasion and non-responders to CRT as compared to patients without bone invasion and responders (p=0.032 and p=0.033, respectively). Multivariate analysis revealed that higher OPG expression was independently associated with shorter cancer-specific survival (p=0.04). The expression status of RANKL and RANK was not significantly related to clinicopathological characteristics and had no impact on survival of OSCC patients. CONCLUSION: Upregulation of OPG expression is associated with bone invasion, poor pathological tumor regression to neoadjuvant CRT, and worse long-term cancer-specific survival in patients with locally advanced OSCC. Our results indicate that OPG may be a novel prognostic biomarker in oral cancer.
Authors: Jonas Eichberger; Florian Weber; Gerrit Spanier; Michael Gerken; Stephan Schreml; Daniela Schulz; Mathias Fiedler; Nils Ludwig; Richard Josef Bauer; Torsten Eugen Reichert; Tobias Ettl Journal: Cancers (Basel) Date: 2022-08-22 Impact factor: 6.575
Authors: Daniel Holzinger; Ivan Danilovic; Rudolf Seemann; Gabriela Kornek; Johannes Engelmann; Robert Pillerstorff; Simone Holawe; Amanda Psyrri; Boban M Erovic; Gregory Farwell; Christos Perisanidis Journal: PLoS One Date: 2016-06-30 Impact factor: 3.240